Productive Replication of Ebola Virus Is Regulated by the c-Abl1 Tyrosine Kinase by Mayra García, Arik Cooper, Wei Shi, William Bornmann, Ricardo Carrion,

Slides:

Advertisements

Similar presentations

Therapeutic Intervention of Ebola Virus Infection in Rhesus Macaques with the MB-003 Monoclonal Antibody Cocktail by James Pettitt, Larry Zeitlin, Do H.

Advertisements

T Cells with Chimeric Antigen Receptors Have Potent Antitumor Effects and Can Establish Memory in Patients with Advanced Leukemia by Michael Kalos, Bruce.

Conserved Shifts in the Gut Microbiota Due to Gastric Bypass Reduce Host Weight and Adiposity by Alice P. Liou, Melissa Paziuk, Jesus-Mario Luevano, Sriram.

Successful Treatment of Ebola Virus–Infected Cynomolgus Macaques with Monoclonal Antibodies by Xiangguo Qiu, Jonathan Audet, Gary Wong, Stephane Pillet,

by Simon Akerman, and Peter J. Goadsby

Targeting Robo4-Dependent Slit Signaling to Survive the Cytokine Storm in Sepsis and Influenza by Nyall R. London, Weiquan Zhu, Fernando A. Bozza, Matthew.

Hypoxia-inducible regulation of placental BOK expression by Daochun Luo, Isabella Caniggia, and Martin Post Biochem. J. Volume 461(3): August 1,

Immune Parameters Correlate with Protection Against Ebola Virus Infection in Rodents and Nonhuman Primates by Gary Wong, Jason S. Richardson, Stéphane.

First-in-Human Testing of a Wirelessly Controlled Drug Delivery Microchip by Robert Farra, Norman F. Sheppard, Laura McCabe, Robert M. Neer, James M. Anderson,

Scanning ultrasound removes amyloid-β and restores memory in an Alzheimer’s disease mouse model by Gerhard Leinenga, and Jürgen Götz Sci Transl Med Volume.

The Effect of Diet on the Human Gut Microbiome: A Metagenomic Analysis in Humanized Gnotobiotic Mice by Peter J. Turnbaugh, Vanessa K. Ridaura, Jeremiah.

Development of Personalized Tumor Biomarkers Using Massively Parallel Sequencing by Rebecca J. Leary, Isaac Kinde, Frank Diehl, Kerstin Schmidt, Chris.

Tumor-derived circulating endothelial cell clusters in colorectal cancer by Igor Cima, Say Li Kong, Debarka Sengupta, Iain B. Tan, Wai Min Phyo, Daniel.

Fig. 1. TP is highly expressed in myeloma.

Fig. 2. Effects of AZD4785 on proliferation and MAPK pathway signaling in KRAS mutant and wild-type cancer cells in vitro. Effects of AZD4785 on proliferation.

Ubiquitination of Drp1 by MARCH‐V.

Transfer of miR-223 during neutrophil-epithelial cell interactions

Fig. 4. Intramuscular injection of AAV9-Cas9/sgRNA-51 corrects dystrophin expression. Intramuscular injection of AAV9-Cas9/sgRNA-51 corrects dystrophin.

A FOXO3a-BIM cascade mediates sensitivity to PARP and MEK inhibition

BET inhibition and depletion repress the expression of BRCA1 and RAD51

Fig. 2. Pharmacologic inhibition of ALK impairs STING activation.

Fig. 8. Gene and protein changes in ALK-dependent STING pathways in human sepsis. Gene and protein changes in ALK-dependent STING pathways in human sepsis.

β-ARs signal cooperatively with mutant EGFR and inactivate LKB1

Proline supplementation during P5CS protein knockdown suppressed GCN2 activation. Proline supplementation during P5CS protein knockdown suppressed GCN2.

Fig. 3. Frequencies of amino acids at critical PGT121 and contact sites in the SHIV-SF162P3 challenge stock. Frequencies of amino acids at critical.

Fig. 2. GPC3 expression in normal and tumor tissues.

Expression of CD36 and psap in a TMA of human ovarian cancer patients

* * * A B D C E CP110 Tubulin WB: CP110 WB: HA CP110 KRAS Tubulin

Fig. 6. HERV-K activation by TDP-43 and identification of binding sites on LTR. (A and B) Stem cell–derived neurons were transfected with either pcDNA.

Fig. 3 Biological function of TG2 and the interaction with MT-2.

Fig. 4. MATE1 transcription in RCC.

Fig. 6 ROC curves of mCCNA1 and mVIM assayed on esophageal balloon samplings of the distal esophagus. ROC curves of mCCNA1 and mVIM assayed on esophageal.

Persistence of CAR4 cells is reduced after sustained TCR engagement

Fig. 1. mGlu7 expression is reduced in RTT autopsy samples.

Fig. 2. STUB1 destabilizes and ubiquitinates TF and mediates TF regulation by AHR. STUB1 destabilizes and ubiquitinates TF and mediates TF regulation by.

Fig. 1. Map showing the study catchment area in the East of England.

Fig. 7. Inhibitory mechanisms of C12G6.

Fig. 3. ERRα is involved in the regulation of OPLAH.

Fig. 2 Fas controls IL-1RA–sEV secretion in murine MSCs.

Fig. 5. Nutlin-3 treatment rescues the proliferation and differentiation of NPCs in vitro. Nutlin-3 treatment rescues the proliferation and differentiation.

Fig. 1. β-APP overexpression or exposure to inflammatory mediators induces sIBM-like pathology in cultured rat myocytes that is abrogated by arimoclomol.

Signaling induced by BRAF L597R and L597S is sensitive to BRAF and MEK inhibitors. Signaling induced by BRAF L597R and L597S is sensitive to BRAF and MEK.

Fig. 2. IL-2/rapamycin–expanded T cells express homing receptors to traffic to lymphoma sites and are resistant to SN-38 toxicity. IL-2/rapamycin–expanded.

Fig. 4. The effect of single-dose rozanolixizumab on the concentration of IgG subtypes in healthy subjects. The effect of single-dose rozanolixizumab on.

Fig. 1. In vivo fates of patient-derived AML cells defined by mutational profile. In vivo fates of patient-derived AML cells defined by mutational profile.

Fig. 3. HECTD2 contains a naturally occurring polymorphism at A19, which mislocalizes to the cytosol. HECTD2 contains a naturally occurring polymorphism.

Bexarotene is neuroprotective in mouse and human HD neurons in vitro

Tyrosine phosphorylations of both DAB1 and ABL are essential for invasion of colorectal cancer cells. Tyrosine phosphorylations of both DAB1 and ABL are.

Fig. 5 Early and modest immune response at day 3 after exposure in Delayed animals. Early and modest immune response at day 3 after exposure in Delayed.

Fig. 1 LB100 and LB102 specifically inhibit PP2A phosphatase activity and the growth of BCR-ABL+ cells. LB100 and LB102 specifically inhibit PP2A phosphatase.

Fig. 4 ALRN-6924 inhibits cellular proliferation and clonogenic capacity, and induces cell cycle arrest and apoptosis in AML cell lines. ALRN-6924 inhibits.

Nilotinib inhibits DDR1 invasive activity of patient‐derived CRC cells

ONC201 activates the ATF4 pathway through the eIF2α kinases HRI and PKR. ONC201 activates the ATF4 pathway through the eIF2α kinases HRI and PKR. (A) Western.

eIF5A-PEAK1 signaling regulates KRAS protein expression.

Fig. 1. Schematic showing the shedding of tumor DNA from head and neck cancers into the saliva or plasma. Schematic showing the shedding of tumor DNA from.

Silver-stained gel showing the major tyrosine-phosphorylated proteins in podocytes. Silver-stained gel showing the major tyrosine-phosphorylated proteins.

Synergistic WNT1 and WNT7B signaling is downstream of LRP6 phosphorylation and β-catenin stabilization. Synergistic WNT1 and WNT7B signaling is downstream.

Effect of the siRNA-mediated knockdown of endogenous MARCH8 on the expression levels of MARCH8 substrates, TfR and CD98, in HepG2 cells. Effect of the.

CO inhibits H2S generation through sGC-dependent cGMP production.

BCL-3 regulates expression of stemness-associated Wnt targets.

Vps36 interacts with Smo in the absence of Hh

GPR124 is the receptor for WNT7B responsible for synergistic signaling

Effect of GSK-3β inhibition on cell-confluence-induced apoptosis.

The microchip-based drug delivery device and overview of study design

A, IGFBP-3 knockdown via siRNA using real-time qRT-PCR analysis.

Fig. 5. miR p inhibits the translational initiation of CACNA1A IRES–driven α1ACT by eIF4AII and eIF4GII. miR p inhibits the translational initiation.

Knockdown of ERBB3, NRG1, or ERBB2 in H358-TwistER cells reduces basal PI3K–AKT signaling and ablates serum-independent proliferation before EMT. A and.

Fig. 4 Dcr-2 binds to the 3′UTR of Toll mRNA.

Fig. 2 Increasing KLF17, CDH1, and LASS2 expression reduced malignant progression and promoted apoptosis of tumor cells. Increasing KLF17, CDH1, and LASS2.

Fig. 3. Association between peak CTL019 expansion and response.

Presentation transcript:

Productive Replication of Ebola Virus Is Regulated by the c-Abl1 Tyrosine Kinase by Mayra García, Arik Cooper, Wei Shi, William Bornmann, Ricardo Carrion, Daniel Kalman, and Gary J. Nabel Sci Transl Med Volume 4(123):123ra24-123ra24 February 29, 2012 Published by AAAS

Fig. 1 Effect of c-Abl1 knockdown and kinase inhibition on Ebola VLP release in transfected 293T cells. Mayra García et al., Sci Transl Med 2012;4:123ra24 Published by AAAS

Fig. 2 Toxicity analysis, morphology of Ebola intracellular nucleocapsids, and nucleocapsid formation. Mayra García et al., Sci Transl Med 2012;4:123ra24 Published by AAAS

Fig. 3 c-Abl1–specific siRNA and nilotinib effects on VP40 VLP egress. 293T cells were transfected with plasmid encoding VP40. Mayra García et al., Sci Transl Med 2012;4:123ra24 Published by AAAS

Fig. 4 Effect of c-Abl1 expression on VP40 phosphorylation and sensitivity to TK antagonist inhibition and siRNA. (A) Western analysis of transfected cell lysates (lanes 1 to 4) or VP40 immunoprecipitates (IP) (lanes 5 to 8) of cells transfected with empty vector control (lanes 1, 3, 5, and 7) or wild-ty Mayra García et al., Sci Transl Med 2012;4:123ra24 Published by AAAS

Fig. 5 Localization of VP40 c-Abl1–mediated tyrosine phosphorylation and effect of tyrosine mutation on VLP release. Mayra García et al., Sci Transl Med 2012;4:123ra24 Published by AAAS

Fig. 6 Effect of siRNA knockdown and c-Abl1 TK inhibition on Ebola virus replication. Mayra García et al., Sci Transl Med 2012;4:123ra24 Published by AAAS