STANDARD PLATE COUNT PCA or NA growth of wide range of microorganisms not exacting in their nutrition requirements fastidious organisms may not grow colonies.

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Presentation transcript:

STANDARD PLATE COUNT PCA or NA growth of wide range of microorganisms not exacting in their nutrition requirements fastidious organisms may not grow colonies may not be from 1 organism sample may not be homogenous enumerates low numbers of organisms

Standard Plate Count Pour Plate method AS (SupercedesAS ) Pour plate method: 1 ml sample + molten agar. NB. Heat of molten agar can cause thermal shock

Colony count - pour plate method

Calculation This is the traditional calculation. The current standard calculation uses two serial dilution counts. A handout in class will explain this further.

Methods: Count colonies in this pour plate. Calculate :- number of colony forming units (cfu) per gram or ml of the food. This is a count of pasteurised milk, therefore the result will be reported as “no. colonies forming units per ml”. If the sample is a solid food item the result will be “cfu per gram”. Which plate will you count? Why? What is the formula for the count of a pour plate?

Extract from AS – Food Microbiology Colony Count - Pour Plate Method See the following for explanations regarding further explanation of counting of colonies and calculations: AS – Counting of colonies ISO Calculations