Draw a reaction map of glucose completely metabolized to CO 2 + H 2 O in the presence of oxygen25 Points Reference: Under: Biol / Class Materials / Unit 2 / Lecture Slides / Chapter 8 Requiresments: 1.Chemical formula; 2.Enzymes, co-enzymes, and co-products;15 points 3.Calculate ATP formation through aerobic metabolism. Adding up of ATPs from four courses: 1.direct formation by a reaction 2.from GTP 3.from NADH 4.from FAD2 5 points 4.Trace C1 of glucose through the pathway as far as you can. 5 points Bonus (2 points): If C1 of glucose is labeled, how many rounds of Krebs Cycle do you expect it to take for it to be completely released as CO 2 ?

Glucose Glucose-6-Phosphate G6P Fructose-6-Phosphate F6P Fructose-1,6-bisphosphate Dihydroxy acetone Glyceraldehyde 3 phosphate 1,3 bisphosphoglycerate Phosphoglycerate kinase 3-Phosphoglycerate2-Phosphoglycerate Phosphoenopyruvate Enopyruvate Pyruvate CYTOSOL MITOCHONDRION Pyruvate Pyruvate Kinase (SPONTANEOUS) C -OH O C=O CH 3 Glyceraldehyde 3 phosphate dehydrogenase Mutase Enolase Aldolase Isomerase Hexokinasep-hexose isomerasep-fructokinase

Pyruvate MITOCHONDRION Acetyl CoA CITRIC ACID CYCLE CoA C -OH O C=O CH 3 CETATE Oxaloacetate CETATE Citrate Isocitrate  -Ketoglutarate Succinyl-CoA Succinate Fumarate Malate Pyruvate dehudrogenase complex Citrate synthase Aconitase Isocitrate dehydrogenase  -Ketoglutarate dehydrogenase SuccinylCoA synthase SuccinylCoA dehydrogenase Fumarase Malate dehydrogenase