Applications & Analysis DNA Gel Electrophoresis 1.

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Presentation transcript:

Applications & Analysis DNA Gel Electrophoresis 1

Preparing DNA Samples for Gel Electrophoresis 1. DNA is mixed with special enzymes 2. Enzymes cut apart the DNA in specific places forming different sized fragments 2

Preparing DNA Samples for Gel Electrophoresis Extraction 1. Cells are isolated from biological specimen such as blood, saliva, urine, semen, and hair 2. The cells are disrupted to release the DNA 3. The DNA can be extracted from the cell nucleus 3

Preparing DNA Samples for Gel Electrophoresis Electrophoresis o DNA samples are placed in gels through which electronic currents are passed o DNA fragments line up in bands along the length of each gel 4

Electrophoresis o An electrophoresis apparatus running five sample of DNA o Arrows show the movement of the negatively charged DNA fragments through the gel matrix 5

Probes o DNA probes identify the unique sequences in a person’s DNA are made up of different synthetic sequences of DNA bases complimentary to the DNA strand bind to complimentary bases in the strand o In most cases, 6 to 8 probes are needed to confirm results 6

Analysis of DNA and Applications Bands and widths are significant in matching samples of DNA 7

Analysis of DNA and Applications o DNA Gel Electrophoresis can determine maternity, paternity, or match to another relative identify human remains match crime scene DNA with a suspect 8