Electrophoresis By John Darwin, Wes Gallagher, and Thompson Grant

History of Electrophoresis Generally known as the electrokinetic phenomenon It was discovered in Russia by a man named Reuss in 1809 It is the motion of dispersed particles relative to a fluid under the influence of an electric field that is space uniform

Electrophoresis in Forensic Science Gel Electrophoresis for: 1.Map the order of restriction fragments within chromosomes 2.To analyze DNA variation within a population by restriction fragment length polymorphisms 3. To determine the nucleotide sequence of a piece of DNA

Process The gel is made and then the molecules are moved through the gel by the electromotive force. After the molecules have been moved through the gel, the gel is stained to make the molecules visible. Ethidium Bromide is used to stain the molecules The molecules form parallel lines and separate from each other and the size shows the molecule’s weight

Applications Used in forensics, molecular biology, genetics, microbiology, and biochemistry Results can be seen with UV light and a gel imaging device The results can give DNA identification

DNA Electrophoresis The identification is used by the different proteins and nucleic acids separating by size The results are shown on a device called an electropherogram

Sources horesishttp://en.wikipedia.org/wiki/Gel_electrop horesis sishttp://en.wikipedia.org/wiki/Electrophore sis phoresishttp://en.wikipedia.org/wiki/DNA_electro phoresis ch/gel/