0 Dpa Control pI 4-7 (Linear) 170 kDa 130 95 72 55 43 34 26 17 10 Biotic stress pI 4-7 (Linear) 170 kDa 130 95 72 55 43 34 26 17 10 170 kDa 130 95 72 55.

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0 Dpa Control pI 4-7 (Linear) 170 kDa Biotic stress pI 4-7 (Linear) 170 kDa kDa Dpa 5 Dpa (a) (b) (c) Supporting Figure 1 Figure S1: 2D-PAGE profile of control (uninfected) and bollworm infested cotton boll proteome during developmental stages. 2D-PAGE profiles of total proteins obtained from control and bollworm infested (Biotic stress) cotton bolls: (a) 0 dpa, (b) 2 dpa cotton bolls, (c) 5 dpa cotton bolls. Equal amount of total proteins (500 µg) were loaded onto 13cm IPG strips, pI 4-7 and protein samples were resolved using 12% SDS-PAGE gels.

Transcriber locus (Ghi _A1_at) Trypsin and protease inhibitor (GhiAffx S1.at) Polygalacturonase (Ghi S1_at) POD9 (Ghi S1_at) Predicted protein (GhiAffx S1_at) Unknown protein (GhiAffx A1_s_at) Transcribed locus (Ghi S1_at) ACS1 (Ghi.23.4.A1_at) Unknown (GhiAffx A1_at) Transcribed locus (Ghi S1_at) Unknown protein (Ghi S1_at) YLS9 (Ghi A1_s_at) Unknown protein (Ghi S1_at) Transcribed locus (Ghi A1_at) Zinc Finger (GhiAffx S1_at) Unknown (Ghi A1_at) Unknown protein (Ghi A1_at) RD26 (Ghi S1_s_at) Transcribed locus (GhiAffx S1_at) ATBCB (Ghi S1_X_at) Unknown (GhiAffx A1_at) Transcribed locus (Ghi S1_at) Weakly similar to (Ghi.4.1.A1_at) ATOSM34 (Ghi S1_at) ABA responsive protein related (Ghi A1_at) Cytochrome P450 (Ghi A1_at) EDGP (Ghi S1_s_at) WD-40 (Ghi SD1_at) Protein kinase family Protein (GhiAffx A1_at) Unknown (Ghi S1_x_at) Unknown (GhiAffx S1_a_at) Unknown (GhiAffx S1_a_at) Supporting Figure 2

0 dpa BS vs CN 2 dpa BS vs CN 5 dpa BS vs CN 10 dpa BS vs CN 0 dpa BS vs CN 2 dpa BS vs CN 5 dpa BS vs CN 10 dpa BS vs CN ATLP-1 (Ghi S1_s_at) Glyoxal oxidase related (Ghi S1_s_at) GBF6 (Ghi A1_at) THI1 (Gra A1_at) Delta 8 sphingolipid desaturase (Ghi S1_at) Phosphate-responsive 1 family protein (Ghi S1_s_at) PGIPL (Ghi S1_s_at) Gibberellin-regulated family protein (Ghi S1_s_at) ATEXPA4 (Ghi A1_at) Unknown (Ghi S1_s_at) 0 dpa BS vs CN 2 dpa BS vs CN 5 dpa BS vs CN 10 dpa BS vs CN 0 dpa BS vs CN 2 dpa BS vs CN 5 dpa BS vs CN 10 dpa BS vs CN Supporting Figure 2 Figure S2: Validation of microarray and proteome data using qRT-PCR during boll development stages (0, 2, 5 and 10 dpa) of cotton under biotic stress. Y-axis represents the log 2 fold change values at various stages in the biotic stress as compared to their respective stages in control.

Biosynthetic process 22% Metabolic process 43% Cellular process 35% Catalytic activity 33% Nucleotide binding 67% Intracellular organelles 42% Cytoplasm 58% (A) Biological process (B) Cellular component (C) Molecular function Supporting Figure 3 Environmental stress Biotic stress Perception of stress eg: Pathogen recognition receptors Signal transduction MAP kinase cascades ROS accumulation Hormone signaling Induction of multiple and individual stress induced transcription factors eg: HSF, AP2/ERF, WRKY, NAC, MYB, AREB/ABF, DREB/CBF, Zinc Finger. Post translational regulation of TF’s Expression of functional downstream response genes. eg: LEA proteins, Heat shock proteins, Ion channels, Genes involved in lignin and secondary metabolites biosynthesis, ROS detoxification, stomatal closure, growth regulation, cell death. Post translational regulation eg: by small RNAs Stress Tolerance/Resistance Figure S3: Gene ontology based classification of overlapping genes in transcriptome and proteome datasets under Biological process (A), Cellular Component (B) and Molecular function categories (C). Key events in the signal transduction pathway activated in response to biotic stress (D) (D)

Figure S4 Changes in gene expression pattern observed in bollworm infested cotton bolls. Genes with experimental indication of involvement in the biotic stress are gathered on the main panel (coloured with dark grey), while genes and pathways that are putatively involved in biotic stress pathway are shown on the left and right sides (coloured in light grey). a) 0 dpa, b) 2 dpa, c) 5 dpa and 10 dpa. In all cases, the signal after infection is expressed as a ratio relative to the signal in unifected controls, converted to a log 2 scale, and displayed. The scale is shown in the figures. A B C D Supporting Figure 4