1 EMBL Outstation — The European Bioinformatics Institute Mus musculus - a model organism in SWISS-PROT

2 EMBL Outstation — The European Bioinformatics Institute SWISS-PROT F Curated protein sequence data bank established in 1986 by Amos Bairoch in Geneva and maintained collaboratively with EMBL since 1987 F Contains currently > protein sequence entries (Release 36)

3 EMBL Outstation — The European Bioinformatics Institute Distinguishing features F High level of annotation F Minimal redundancy F High level of integration with other databases

4 EMBL Outstation — The European Bioinformatics Institute TrEMBL F Computer-annotated supplement to SWISS-PROT F Consists of entries in SWISS-PROT format F Contains translations of all coding sequences in EMBL Nucleotide Sequence Database which are not yet in SWISS-PROT F Consists of SP-TrEMBL and REM-TrEMBL

5 EMBL Outstation — The European Bioinformatics Institute Model organisms in SWISS-PROT F Target of genome sequencing and/or mapping F Priority annotation u incorporate new sequences/updates as quickly as possible u high level of annotation u cross-references to specialised databases u additional information in form of specific documents

6 EMBL Outstation — The European Bioinformatics Institute Current status of mouse sequences F Total of 7006 entries in SWISS-PROT and TrEMBL F 3264 entries in SWISS-PROT F 3742 in SP-TrEMBL

7 EMBL Outstation — The European Bioinformatics Institute Annotation F Citation information F Taxonomic data F Sequence data F Function(s) of the protein F Post-translational modification(s) F Domains and sites F Secondary and quaternary structure F Similarities to other proteins F Disease(s) associated with deficiencie(s) in the protein F Sequence variants, conflicts

8 EMBL Outstation — The European Bioinformatics Institute Annotation sources F Publications reporting sequence data F Review articles F External experts

9 EMBL Outstation — The European Bioinformatics Institute Integration with other databases F Sequence u EMBL Nucleotide Sequence Database F Structure u Protein Data Bank (PDB)

10 EMBL Outstation — The European Bioinformatics Institute Integration F Specialised data collections u e.g. ENZYME, PROSITE u Mouse Genome Database (MGD) u Index of MGD entries referenced in SWISS-PROT:

11 EMBL Outstation — The European Bioinformatics Institute ID GCDH_MOUSE STANDARD; PRT; 438 AA. AC Q60759; DT 01-NOV-1997 (REL. 35, CREATED) DT 01-NOV-1997 (REL. 35, LAST SEQUENCE UPDATE) DT 01-NOV-1997 (REL. 35, LAST ANNOTATION UPDATE) DE GLUTARYL-COA DEHYDROGENASE PRECURSOR (EC ). GN GCDH. OS MUS MUSCULUS (MOUSE). OC EUKARYOTA; METAZOA; CHORDATA; VERTEBRATA; TETRAPODA; MAMMALIA; EUTHERIA; RODENTIA. RN [1] RP SEQUENCE FROM N.A. RC STRAIN=129/SV; TISSUE=LIVER; RX MEDLINE; RA KOELLER D.M., DIGIULIO K.A., ANGELONI S.V., DOWLER L.L., FRERMAN F.E., WHITE R.A., GOODMAN S.I.; RL GENOMICS 28: (1995). CC -!- CATALYTIC ACTIVITY: GLUTARYL-COA + ACCEPTOR = CROTONOYL-COA + CO(2) + REDUCED ACCEPTOR. CC -!- COFACTOR: FAD FLAVOPROTEIN. CC -!- PATHWAY: DEGRADATIVE PATHWAY OF L-LYSINE, L-HYDROXYLYSINE, AND L-TRYPTOPHAN METABOLISM. CC -!- SUBUNIT: HOMOTETRAMER. CC -!- SUBCELLULAR LOCATION: MITOCHONDRIAL MATRIX. CC -!- SIMILARITY: BELONGS TO THE ACYL-COA DEHYDROGENASES FAMILY. DR EMBL; U18992; G ; -. DR MGD; MGI:104541; GCDH. DR PROSITE; PS00072; ACYL_COA_DH_1; FALSE_NEG. DR PROSITE; PS00073; ACYL_COA_DH_2; 1. KW OXIDOREDUCTASE; FLAVOPROTEIN; FAD; MITOCHONDRION; TRANSIT PEPTIDE. FT TRANSIT 1 44 MITOCHONDRION (POTENTIAL). FT CHAIN GLUTARYL-COA DEHYDROGENASE. SQ SEQUENCE 438 AA; MW; 8C9149C3 CRC32;

12 EMBL Outstation — The European Bioinformatics Institute Summary F Complete with minimal redundancy F As much up-to-date information as possible on each sequence F Priority annotation of mouse sequences F MGD cross-refererences

13 EMBL Outstation — The European Bioinformatics Institute SWISS-PROT at EBI F Rolf Apweiler F Sergio Contrino F Wolfgang Fleischmann F Henning Hermjakob F Vivien Junker F Stephanie Kappus F Fiona Lang F Michele Magrane F Maria Jesus Martin F Nicoletta Mitaritonna F Steffen Moeller F Claire O’Donovan

14 EMBL Outstation — The European Bioinformatics Institute Some ways to access SWISS-PROT + TREMBL F ftp.ebi.ac.uk/pub/databases F F F F