海洋天然物 Elysia rufescens 海蛞蝓 Kahalalide F (KF) 指導老師:詹于誼 組員:楊仲智 張芳瑛 翁曼真
0.2 to 4 M using a continuous 96 hr(median = 0.75 ).
Images of cells treated with KF 5 M for PLC/PRF/5C 0.5 M for HepG2 KFcontrol
4–6 M KF in PLC/PRF/5C cell 5M5M 2.5 M 1M1M 0.5–0.7 M KF in HepG2 cells 0.1 M 1M1M 2.5 M 5M5M 0.5 M 10 M 25 M KF-induced depletion of ATP in HepG2 and PLC/PRF/5C cells
Analysis of KF-induced permeability changes in HepG2 cells by flow cytometry (a) control
(b) treated with 0.25 M KF for 1 h
LDH release by HepG2 and PLC/PRF/5C after exposure to KF for 1 h. HepG2 PLC/PRF/5C
Discussion HepG2 cells demonstrated profound ATP depletion, associated with cell swelling and cell blebbing, and increased permeability to propidium iodide (PI), annexin V (AV) and release of lactate dehydrogenase (LDH).cell membrane induced by KF in HepG2 PLC/PRF/5C cells retained their cell structure, but were permeable to PI and, following exposure to high concentrations of KF, to AV. PLC/PRF/5C suggest specific interactions with membranes or proteins. This could lead to better drug design aimed at exploiting the potential for cell selectivity.