Moving Green Fluorescent Protein

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Presentation transcript:

Moving Green Fluorescent Protein A jellyfish gene is identified that codes for a protein (GFP) that glows under UV light. Researchers clone the gene and insert it into bacteria.

Green Fluorescent Protein Plasmid from E. coli amp resistance ara B ara A ara D P Green Fluorescent Protein O ara C Gene that codes for the inhibitor protein Operator for the arabinose operon. Gene that gives bacteria resistance to the antibiotic ampicillin. Promoter for the arabinose operon. Structural genes for arabinose metabolism

Function of the GFP gene in pGLO GREEN FLUOESCENT PROTEIN Repressor protein (from ara C gene) O P GREEN FLUOESCENT PROTEIN GFP gene (from jellyfish) Regulatory genes (operator & promoter)

Function of the GFP gene in pGLO GREEN FLUOESCENT PROTEIN RNA polymerase O P GREEN FLUOESCENT PROTEIN

Function of the GFP gene in pGLO GREEN FLUOESCENT PROTEIN Arabinose O P GREEN FLUOESCENT PROTEIN

Function of the GFP gene in pGLO GREEN FLUOESCENT PROTEIN Arabinose O P GREEN FLUOESCENT PROTEIN

Function of the GFP gene in pGLO GREEN FLUOESCENT PROTEIN Arabinose Arabinose O P GREEN FLUOESCENT PROTEIN

Function of the GFP gene in pGLO GREEN FLUOESCENT PROTEIN RNA polymerase Arabinose O P GREEN FLUOESCENT PROTEIN

Function of the GFP gene in pGLO GREEN FLUOESCENT PROTEIN RNA polymerase Arabinose O P mRNA GREEN FLUOESCENT PROTEIN