Cox1 Barcoding of Penicillium and Fusarium Keith A. Seifert, Scott Gilmore and Karol Rivera Biodiversity, Agriculture & Agri-Food Canada, Ottawa, Ontario,

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Presentation transcript:

Cox1 Barcoding of Penicillium and Fusarium Keith A. Seifert, Scott Gilmore and Karol Rivera Biodiversity, Agriculture & Agri-Food Canada, Ottawa, Ontario, Canada. Agriculture and Agri-Food Canada Agriculture et Agroalimentaire Canada Canada

Gene 1 tree

T 474 Outgroup T T 478 Outgroup T 483 Outgroup T 475 Outgroup T 485 Outgroup T 486 Outgroup T 488 Outgroup T 487 Outgroup T 354 Taxon T 352 Taxon T L Taxon T 357 Taxon T 066 Taxon T 068 Taxon T T 071 Taxon 2 T 342 Taxon T 347 Taxon T 348 Taxon change Taxon 3 complex Gene 2 Taxon 3 Taxon 2 Taxon 1 Cox1 Taxon 1 Taxon 2 Taxon 3 Gene 1

beta-tubulin Cox1 Taxon 1 Taxon 2 Taxon 3 Taxon 4 Taxon 5 Taxon 6 Taxon 7 Taxon 8 Gene 1 tree Gene 2 tree

Comparison of Three Potential Barcoding Loci Gene 1Gene 2Gene 3 Mean bp Infraspecific range Interspecificx range Species resolution Or PCR success rate95%75%95% Introns030 Gaps

Comparison of Three Potential Barcoding Loci in Penicillium subgenus Penicillium Cox1BenAITS Mean bp Infraspecific range Interspecificx range Species resolution Or PCR success rate95%75%95% Introns030 Gaps

Neighbour joining analysis of Cox1 exon region between Fusarium intron 4b and end of barcode (439bp).

Hypocreales – Primer Design AHyFu-F FI3-F FI3-R AHyFu-R FI4b-F

Acknowledgements This work was funded by grants to the Canadian Barcode of Life Network by –The Natural Sciences and Engineering Research Council of Canada (NSERC) –Genome Canada