Genetic Engineering Genetic engineering involves the manipulation of organism genes. The central tool is the recombinant DNA technique. - to isolate a.

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Presentation transcript:

Genetic Engineering Genetic engineering involves the manipulation of organism genes. The central tool is the recombinant DNA technique. - to isolate a gene of interest from one organism - to insert the isolated gene to a DNA molecule (known as vector) that can replicate. - to provide an environment (known as recipient cells) that allows reproduction of the joined DNA molecule and generate the gene product.

Genetic Engineering To transfer a gene from one organism to another - transformation: plasmid as vector - transduction: viruses as vector - conjugation: direct cell-to-cell contact is required. The gene is transferred by an episome (DNA molecule).

Transformation Transfer and cloning of insulin gene

Transformation Transfer and cloning of insulin gene bacterial DNA