BIOE/CHBE 460 Biochemical Engineering. Early penicillin culture facility at the Sir William Dunn School of Pathology, Oxford, England. © Museum of the.

Slides:

Advertisements

Similar presentations

Applications of Biotechnological Processes Antibody Production.

Advertisements

antibodies produced by differentiated B-cells

5 Stages involved in GE Isolation Cutting Ligation and Insertion

Ameer Effat M. Elfarash Dept. of Genetics Fac. of Agriculture, Assiut Univ. From Gene to Protein (an overview)

Lecture 3 Problem: PromoterCoding Region ORF deleted protein You have cloned a new bacterial gene encoding enzyme X, sequenced the DNA, and deduced the.

Recombinant DNA Technology

Biotechnology Chapter 11.

Fundamentals of Biotechnology

Monoclonal Antibodies

Chapter 4: recombinant DNA

Key Area : Genetic Control of Metabolism in Micro-organisms Unit 2: Metabolism and Survival.

Recombinant DNA Technology “Gene Cloning”. What is it?  Gene cloning: production of large quantities of a specific, desired gene or section of DNA to.

Monoclonal Antibodies. Antibodies have important uses beyond fighting infections in the body. Production of long-lasting monoclonal antibodies is a recent.

Genetic Engineering Biotechnology Molecular Cloning Recombinant DNA.

Hybridoma Technique.

Biopharmaceutical Products Touqeer Ahmed Ph.D. Atta-ur-Rahman School of Applied Bioscience, National University of Sciences and Technology 23 rd September,

Monoclonal Antibody Production Cathy Langford, Judy Knadler, Jamie Wolf.

Monoclonal Antibodies BAT: Explain what monoclonal antibodies are and discuss their uses.

Production of Monoclonal antibodies

“MONOCLONAL ANTIBODIES” Jony Mallik M

11.1 Defense Against Infectious Disease. Process of blood clotting When small vessels burst, blood escapes into your surrounding tissues (often these.

Monoclonal antibodies Hybridoma Technique. Monoclonal antibodies (mAb or moAb) Monoclonal antibodies are:  monospecific antibodies that are identical.

The production procedure Step 1 Hybridoma cell production Hybridoma cell( 杂交瘤细胞 ): the cell which made by fusing a specific antibody-producing B cell with.

Lec 16 Medical biotechnology Shah Rukh Abbas, PhD

6.2 Genetic Engineering. Genetic Engineering Altering the sequence of DNA molecules Important in developing drugs Insulin  Human insulin produced by.

THE BASIS FOR TRANSGENIC ORGANISMS. TRANSFORMATION The incorporation of a piece of naked DNA (not attached to cells) from one organism into the DNA of.

PRINCIPLES OF BIOTECHNOLOGY

ABDUALLAH SAUD AL-SHETELY

Introduction to biotechnology Haixu Tang School of Informatics.

Bioreactor Systems Ka-Yiu San.

Applications of Ab Molecules Chapter 4 Monoclonal Ab (p.104) Chapter 5 Ab genes and Ab Engineering (p.139)

Monoclonal Antibodies Aims: Must be able to define the term monoclonal antibody. Should be able to outline the possible uses of monoclonal antibodies.

Biotechnology and Recombinant DNA

Recombinant DNA Technology Pre-AP Biology 10/5/2015.

Producing drugs using genetically modified organisms.

Chapter 20 Experimental Systems Dr. Capers.  In vivo ○ Involve whole animal  In vitro ○ Defined populations of immune cells are studied under controlled.

Ka-YiuSan Metabolic Engineering and Systems Biotechnology Laboratory.

Microbial Biotechnology Philadelphia University

Monoclonal Antibodies Dr. Aws Alshamsan Department of Pharmaceutics Office: AA87 Tel:

Genetic Engineering. What is genetic engineering? Definition: process of changing an organism’s genetic material to produce a new and useful result This.

Chapter 12 Lecture Outline Molecular Techniques and Biotechnology.

Biotechnology is the use of biological systems, such as microorganisms, whole cells or their molecules, to solve problems or to make useful products.

Recombinant DNA What is the basis of recombinant DNA technology? How does one “clone” a gene? How are genetically modified organisms (GMOs) created? Illustration.

GENETIC RECOMBINATION By Dr. Nessrin Ghazi AL-Abdallat Lecturer of Microbiology.

Studying the genomes of organisms GENE TECHNOLOGY.

Molecular Basis for Relationship between Genotype and Phenotype DNA RNA protein genotype function organism phenotype DNA sequence amino acid sequence transcription.

Genetic Engineering Genetic engineering involves the manipulation of organism genes. The central tool is the recombinant DNA technique. - to isolate a.

A Molecular Toolkit AP Biology Fall The Scissors: Restriction Enzymes  Bacteria possess restriction enzymes whose usual function is to cut apart.

Genetic engineering Lesson Objectives Genetic engineering involves changing the genetic material of an organism Genes can be transferred from one organism.

Gene Cloning & Creating DNA Libraries. Клонирование генов Что означает термин «клонирование»? Как происходит клонирование генов? Чем это отличается от.

KSU Microbiology section Dr. sarah I bukhari Assistant Professor of pharmaceutical microbiology.

- making changes to the DNA code of a living organism.

Title: Genetic Techniques 1

Pharmaceutical Biotechnology

Department of Zoology, R.U.

Monoclonal Antibody Production

Pharmaceutical Biotechnology

Fac. of Agriculture, Assiut Univ.

Chapter 1. The Molecular Biotechnology Revolution

Pharmaceutical Biotechnology

Introduction to the Biotechnology Workplace

Introduction to the Biotechnology Workplace

Genetic Engineering Insulin production Extra-nutrient foods

x Antigen Spleen cells Immunization Cell fusion Monoclonal antibodies

Recombinant Vaccines A recombinant vaccine is a vaccine produced through recombinant DNA technology. This involves inserting the DNA encoding an antigen.

HYBRIDOMA TECHNOLOGY & PRODUCTION OF MONOCLONAL ANTIBODIES

Lec 6 Monoclonal Antibody.

Monoclonal antibody production and application

Presentation transcript:

BIOE/CHBE 460 Biochemical Engineering

Early penicillin culture facility at the Sir William Dunn School of Pathology, Oxford, England. © Museum of the History of Science, Oxford Merck Archives, ©Merck & Co. Inc.

Example of process improvement

Major advancements Molecular biology Genetic engineering Cell and tissue culture

Examples of genetic engineering application (first generation application)

Recombinant proteins by microorganisms Restriction sites Ligation Transformation Cloning vector Recombined plasmid Restriction cleavage Restriction cleavage Gene of interest Transcription Translation mRNA Protein Host cell

Recombinant proteins by microorganisms YearProducts DiseaseCompany 1982Humulin Type 1 diabetesGenetech, Inc. (synthetic insulin) 1985ProtropinGrowth hormone Genetech, Inc. Deficiency Some early products

Examples of pathway and metabolic engineering (second generation application of genetic engineering)

Introduction What is pathway and metabolic engineering?

chilling tolerant plant*

Cell and tissue cultures monoclonal antibody by hybridoma cells

Monoclonal Antibody screen for desired clone only hybridoma cells grow cells grown in HAT medium hybridoma myeloma cells antigen injected into mouse spleen removed B lymphocytes

Applications of Monoclonal Antibodies Diagnostic ELISA Tests – PSA, AIDS, pregnancy Passive Immunization Detection, isolation and purification of biomolecules

by Genentech, Corporate Communication A Fermenter / Bioreactor And Its Parts

Separation

Chromatography Columns BioProcess Glass (BPG) Columns Amersham Pharmacia Biotech

Single System for Anchorage-Dependent and Suspension Cultures New Brunswick Scientific Company