-11.9 kb -4.2 kb -5.6 kb Gfi1 +/1b neo Gfi1 +/+ Gfi1 +/1b Gfi1 1b/1b Gfi1 +/1b Gfi1 +/+ -11.9 kb - 4.2 kb Gfi1 +/+ Gfi1 +/1b -14 kb - 8 kb B A Gfi1 +/+

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Presentation transcript:

-11.9 kb -4.2 kb -5.6 kb Gfi1 +/1b neo Gfi1 +/+ Gfi1 +/1b Gfi1 1b/1b Gfi1 +/1b Gfi1 +/ kb kb Gfi1 +/+ Gfi1 +/1b -14 kb - 8 kb B A Gfi1 +/+ Gfi1 +/1b Gfi1 1b/1b Gfi1 (55 kDa) Gfi1b (37 kDa) loading control C Supp. Figure 1

Gfi1 +/+ Gfi1 P2A/P2A - 15 kb - 8 kb B Gfi1 +/P2A Gfi1 +/ kb - 8 kb Gfi1 +/P2A Gfi1 P2A/P2A Gfi1 +/+ Gfi1 -/- Gfi1 (55kDa) loading control A Supp. Figure 2

∆ Rn cycle Amplification Plot Gfi1 1b/1b E E E E E+1 Gfi1 Gfi1b E E E E E+1 Gfi1 Gfi1b Amplification Plot Gfi1 +/+ A B Gfi1 Gfi1b GAPDH Gfi1 +/+ Gfi1 1b/1b H 2 O Supp. Figure 3

IP: anti Gfi1b We: anti PIAS3 PIAS IP: anti Gfi1b We: anti Gfi1b Input IP anti-GSTIP anti-Gfi1b Gfi1b IgG Supp. Figure 4

Table 1 Differential cell counts of wild type, Gfi1 -/-, Gfi1 P2A/P2A and Gfi1 1b/1b mice Supp. Table 1  significant