T. Trimpe 2005 Base - Single piece of metal -Support here when moving.

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Presentation transcript:

T. Trimpe

Base - Single piece of metal -Support here when moving

Arm -Hold here when moving

Stage - Supports specimen -Hole allows light to pass through

Diaphragm - controls amount of light -ranges 1-5

Stage Clips -secures specimen/slide

Bulb/Mirror - Light source -NEVER use the sunlight it will hurt your eyes!!

Scanning Objective Lens -Shortest lens 4x magnification

Low Power Objective Lens -Medium length lens 10x magnification

High Power Objective Lens -Longest lens 40x magnification

Body Tube - Allows light from objective to pass upward

Eye Piece/Ocular lens -Site of observation 10x magnification

Nose Piece -Allows you to switch objective lenses

Coarse Adjustment Knob - Used to focus specimen -DO NOT use on high power objective lens

Fine Adjustment Knob -Used for minute focusing -Used after coarse adjustment knob

Total magnification = (ocular lens power) x (objective lens power) Example: (10x)ocular lens x (40x)high power lens = 400x total magnification Magnification: The ability to increase the size of an image Ocular LensObjective Lens = 400x total magnification X

Comparing Powers of Magnification We can see better details with higher the powers of magnification, but we cannot see as much of the image. Which of these images would be viewed at a higher power of magnification?

Light Pathway: Light Source → Specimen → Objective Lens → Body Tube → Eye Piece

Resolution- add this to your notes! making the image clear ability to determine between two objects or points can be adjusted with the fine/course knob or the diaphragm (to allow more light in)

Use of a Scope Carry the microscope with 2 hands (arm & base) Looking at a slide: 1. Use ONLY ONE slide at a time 2. ALWAYS start at LOW power first(10x) 3. ALWAYS start with stage lowered completely 4. Make sure the objective clicks into position 5. The coarse focus knob is ONLY used on the lowest power lens 6. The fine focus knob is used on all lenses to bring the image into focus

Putting the Microscope Away Lower the stage completely and click the lowest objective into place Return slide to tray Wind up the scope power cord

Types of Microscopes 1. Compound Light Microscope: Shines light through a specimen and uses 2 lenses for magnification Magnification – increase in an objects apparent size Resolution – how clear the image appears once magnified Max clear magnification ~2000x 2. Electron microscope: Uses electrons instead of light to visualize the specimen, electrons bounce off or pass through and a computer interprets a picture Always black and white, specimen must be dead to examine Scanning EM – magnifies up to 100,000x Transmission EM – magnifies up to 200,000x

Microscope Images Compound Light Microscope - Protozoa Transmission Electron Microscope - Bacteria Scanning Electron Microscope (SEM) Butterfly Tongue