Tayeesa and Anna Gel Electrophoresis

The process:  Restriction enzymes cut DNA of interest  The pieces of restricted DNA are placed into the wells on the gel.  Then a voltage (current) is placed on the agarose gel, this happens by electrodes on both ends.  The current separates the macromolecules by size and charge. (they move at different speeds depending on size)  Negative moves towards positive end. (Anode)

Visualize the Process:

Blot: -Capillary action pulls an alkaline solution through the gel - transfers DNA through paper - denatures it -single strands of DNA stick to the paper

PCR Denaturing the double strand  polymerase chain reaction  multiplies pieces of DNA  exponential multiplication of DNA  PCR makes enough DNA to do Gel Electrophoresis on

Nylon Filter  Sits on top of gel  filter paper takes up genetic material  process of blot  Basically sucks up the genetic information  Used to make the results readable

Radioactive Probe/ X Ray film  paper plot exposed to solution containing radioactively labeled probe  the probe is a single stranded dna that is complementary to dna sequence of interest  attaches by base pairing to restriction fragments

Problems  If it is not recognized by restriction enzymes:  would be a long band  If a scientist puts it on the wrong side then the gel  would not move  Notice no lines

When would you use gel electrophesis  Visualize DNA  see changes in a person’s DNA  recognize what is not the same between two pieces of DNA  crime scenes

Works Cited electrophoresis&h=270&w=593&tbnid=cfSMX7AMBJVIMM:&docid=9p_2nTcYHGYqfM&ei=QjqvVtigJ4Wp-QGw6JDwBA&tbm=isch&ved=0ahUKEwjYye2VtNbKAhWFVD4KHTA0BE4QMwgyKAAwAA nPtdbKAhXGWT4KHcDJA1AQjRwIBw&url=http%3A%2F%2Fenfo.agt.bme.hu%2Fdrupal%2Fen%2Fnode%2F8926&bvm=bv ,d.cWw&psig=AFQjCNGuVWA- MZ07BjwizNhOB22LQ47dyw&ust= hern_blot&bvm=bv ,d.cWw&psig=AFQjCNEnF_x2Bdd6Xv5Z1ZQsLy_JXS6z_g&ust= Fid1.html&bvm=bv ,d.cWw&psig=AFQjCNF8OYovkCdvYyxee5_mb_RLKcmQwA&ust= questions%2F16290%2Fhow-do-i-get-a-brighter-dna-bands&bvm=bv ,d.cWw&psig=AFQjCNFe8TPNq49HHLrD_bIUb6aTUsasFA&ust= PCR%2520quantitation%2520of%2520cytokines&h=259&w=361&tbnid=FkK8bri71avGPM:&docid=uRaizcX1160IgM&ei=nT6vVtHcDoa3- AHfuYX4BA&tbm=isch&ved=0ahUKEwiRn6upuNbKAhWGGz4KHd9cAU8QMwgtKBEwEQ Our textbook is where we gained most of the knowledge. GO BIO!