Fig. 5.3

Transforms R cells

Fig. 5.5

Uracil (U) Five Fig. 5.7

(a.k.a. polarity) Sequence: 5’ ATGCA 3’

Fig. 5.6

34 Fig. 5.9

34 Fig. 5.9

Fig. 5.14

Models for DNA Replication Daughter Helix: semi-conservative: one old/one new strand conservative: two new strands dispersive: mix of old & new

Fig or

Fig. 5.17

Bacterial Chromosome Eukaryotic Chromosome

Initiation: Opening and Stabilizing the Replication Bubble origin of replication Initiator Protein DNA Helicase Single Stranded Binding Proteins

DNA Polymerase III 10 protein subunits Restrictions on Nucleotide Addition –Copies only single stranded DNA –Can add only to pre-existing chains –Adds only in the 5’ to 3’ direction

Fig. 5.17

AGGCCTCGAATTCGTATAGCTTTCAGA5’3’ UUAAGCAUAU5’3’ WHAT WOULD BE THE RNA PRIMER’S SEQUENCE? Alternatively: 5’ UAUACGAAUU 3’

Elongation Phase of DNA Replication Nucleotide Selection –via complementary base pairing Phosphodiester Bond Forms Helicase Continues Moving –opening the Replication bubble DNA Polymerase Trails Behind –adding more nucleotides

DNA Polymerase III 10 protein subunits Restrictions on Nucleotide Addition –Copies only single stranded DNA –Can add only to pre-existing chains –Adds only in the 5’ to 3’ direction

3’ end5’ end

Origin 5’ 3’ 5’ 3’ leading strand lagging strand movement of growing fork

1. Bidirectional replication 2. Leading Strand Continuous 3. Lagging Strand Discontinuous Okazaki Fragments 5’3’ 5’3’