4.2 (a) To prepare and examine one animal cell, stained and unstained, using the light microscope
Step 1 Swab the inside of the cheek.
Step 2 Transfer the sample to a slide.
Step 3 Cover the sample with a drop of water.
Step 4 Place the cover slip at the edge of the water at a 45° angle.
Step 5 Slowly lower the cover slip onto the water using the mounted needle.
Step 6 Dry the slide if necessary.
Step 7 Examine the slide under the microscope using the usual procedure.
Step 8 To stain: Prepare as for steps 1 and 2 previously
Step 9 Cover the sample with one drop of methylene blue stain.
Step 10 Gently wash excess stain from the slide using a wash bottle.
Step 11 Apply the cover slip as previously done.
Expected result
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