StainsStains. Cheek Cells Without Stain Cheek Cells with Simple Stain (Methylene Blue)

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Presentation transcript:

StainsStains

Cheek Cells Without Stain

Cheek Cells with Simple Stain (Methylene Blue)

Simple Stain: Negative Stain

Differential Stains: Gram Stain Color of Gram + Color of Gram – Primary stain: Crystal violet Purple Mordant: Iodine Purple Decolorizing agent: Alcohol-acetone PurpleColorless Counterstain: Safranin PurplePink

Gram Positive and Gram Negative Bacteria

Prokaryotic Cell Drawing

Outer membrane Peptidoglycan GRAM NEGATIVE GRAM POSITIVE

Capsule Stain

Figure 3.12a-c

Acid-Fast and Non Acid-Fast

Endospore Stain: Ziehl-Neelsen

Figure 3.12a-c

Endospore Stain

Stain Comparisons G+ (PURPLE) G – (PINK) ACID FAST SPORE STAIN PRIMARY STAINCrystal violet Carbol Fuschia Malachite green MORDANTIodine Heat DECOLORIZERETOH or acetone: Cell is purple ETOH or acetone: Cell is clear Acid alcohol Water COUNTER-STAINSafranin: Cell is purple Safranin: Cell is pink Methylene Blue Safranin: Spores are green Cell is pink

Flagella Stain

Figure 3.12a-c

Monotrichous Lophotrichous Amphitrichous Peritrichous Names for flagella arrangement

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