A Study of Endocrine Disrupting Compounds in Wastewater Arthur Ho Spacegrant Mentor: Dr. Bob Arnold Chemical and Environmental Engineering University of.

Slides:

Advertisements

Similar presentations

Home Work Each day 3780 m3 of wastewater is treated at a municipal wastewater treatment plant. The influent contains 220 mg/L of suspended solids. The.

Advertisements

Welcome to Shawnee #2 Wastewater Treatment Plant.

Welcome to American #2 Wastewater Treatment Plant.

Endocrine Disruptor Screening Program: Ralph L. Cooper Endocrinology Branch Reproductive Toxicology Division NHEERL, U.S. EPA Male and Female Pubertal.

The Critical Challenge of Antibiotic Resistance: Are Wastewater Treatment Plants a Concern? Kourtney Brown 1, Stefan Walston 2, Channah Rock 2, & Jean.

Estrogen reduction in a coupled wetland and ground water flow-through system Laura Hanna Eric Peterson Illinois State University.

Monitoring of endocrine disruption in different milieu matrices W. Dhooge , F.H. Comhaire, A. Mahmoud, F. Eertmans, J.M. Kaufman Endocrinology/Andrology,

Toxicological report Bio 464

Determination of Natural Steroidal Estrogens in Flushed Dairy Manure Wastewater and Surface and Ground Water Travis Hanselman Ph.D. exit seminar.

Modeling Advanced Oxidation Processes for Water Treatment Ashley N. Anhalt, A. Eduardo Sáez, Robert G. Arnold, and Mario R. Rojas Department of Chemical.

An Evaluation of Models to Predict the Activity of Environmental Estrogens Candice M. Johnson and Rominder Suri, Ph.D.,P.E. NSF Water and Environmental.

Endocrine Disrupting Compounds: General Overview and Impact on Freshwater Biology SCAP Water Issues Committee Meeting May 5, 2011 Photo by Judy Gibson.

Detection of Estrogenic Hormones in BC Sewage Treatment Plant Effluents Heather Osachoff PhD candidate, Simon Fraser University Toxicogenomics Analyst,

METHODS RESULTS Yuan Li The James Hutton Institute, Aberdeen AB15 8QH, UK; The Robert Gordon University, Aberdeen AB10 7JG, UK; Research Center for Eco-Environmental.

Estrogenic Compounds in Wastewater Presentation to the Metropolitan Council Environment Committee September 23, 2008 Paige Novak, Deb Swackhamer, Mike.

Surface Water Treatment Plant

Determining Estrogenicity of a Cytochrome P450- dependent metabolite of 3,3’-diindolylmethane (DIM) Rachel O’Neal Susan Tilton Dr. David Williams Marine.

June 2010 LANDSIEDEL 1 Chemical Industries Role in Tomorrows Toxicity Testing Robert Landsiedel, Susanne Kolle, Tzutzuy Ramirez, Hennicke Kamp and Ben.

The Effects of Caffeine and Triclocarban on Gammarus pseudolimnaeus Jim Fietzer Department of Biological Sciences, University of Wisconsin – Whitewater.

Welcome to Shawnee #2 Wastewater Treatment Plant.

Welcome to Shawnee #2 Wastewater Treatment Plant.

Nonylphenol in Water By Jackie Fawcett & Michael Goldrich.

Degradation of Hormone Contaminants in Waters by OH Oxidation Katy Swancutt Stephen Mezyk.

Biodegradation of Emerging Contaminents by Pseudomonas butanovora Alisha Norman MacArthur High School Aldine Independent School District Mentor: Kung-Hui.

Biodegradation of trace contaminants – pharmaceuticals and personal care products Dr. Abbie Porter May 3, 2010.

Wastewater Treatment. Collection Sewers Collect wastewater and bring it to the wastewater treatment plant – Combined sewer overflows: Take untreated sewage.

Emerging Contaminants: Fate During Wastewater Treatment and Strategies to Enhance Removal David Quanrud The University of Arizona New Directions in Wastewater.

Lsfdlkdfj ;la;fkd theklekrj Girly Grass Dana Devin-Clarke. Sally Brown, Michael Muramoto*, and Michael Doubrava* University of Washington and *King County.

Hazards and Risks.

The Intact Male Assay As An Alternative Tier I Screening Assay For Detecting Endocrine-Active Compounds John C. O’Connor DuPont Haskell Laboratory for.

Endocrine Disruption in the Marine Environment Mike Waldock Cefas Weymouth Laboratory Barrack Road Weymouth Dorset. UK DT48UB

Water Treatment Chapter 11. Sewage Treatment Rationale More than 500 pathogenic bacteria, viruses, and parasites can travel from human or animal excrement.

Environmental Studies - Spring 2005 HPLC Lab (Environ.- & Occu. Med.) The job and the problem You want to do this by using a test (an assay) that responds.

SEX HORMONES ผศ. พญ. มาลียา มโนรถ. Sex Hormones F 21 carbon : progestin F 19 carbon : androgen F 18 carbon : estrogen.

LOGO ASSESSMENT OF CYTOTOXICITY AND ACUTE TOXCITY OF SELECTED ENDOCRINE DISRUPTING COMPOUNDS COMMONLY PRESENT IN FOOD PRODUCTS Katarzyna Owczarek, Błażej.

Environmental Estrogens Stimulate Gene Transcription in the Prolactin Promoter Danae Fitzmaurice and Winnifred Bryant Ph. D. Department of Biology University.

Thuan Chau EMERGING CONTAMINANTS PHARMACEUTICALS AND PERSONAL CARE PRODUCTS (PPCPs) “Contaminants of Emerging Concern” (CECs) – EPA University of Utah.

Environmental impact assessment of steroid hormones R. Laenge, LGE 09 June 2006 Assessment of the impact of selected steroid hormones on biodiversity Reinhard.

Fate of Chemical and Biological Emerging Contaminants in Biosolids and After Land Application Ian L. Pepper The University of Arizona The Northwest Biosolids.

An Overview of EDC’s, Pharmaceuticals and Personal Care Products in Our Drinking Water Presented By: Roxanne Russell, Yuh-Chi Niou, Kris McArthur, Amelia.

Biodegradation of Emerging Contaminants by Pseudomonas butanorova Shervada Hall Houston Independent School District Empowerment College Preparatory High.

Advisory Panel on Emerging Contaminants Unregulated Chemical Contaminants APEC Update – September 27, 2013.

OPTIMIZATION OF BISPHENOL A, 4-t-OCTYLPHENOL, AND 4-NONYLPHENOL EXTRACTION FROM HUMAN BLOOD SERUM WITH HYBRID SOLID PHASE EXTRACTION – PROTEIN PRECIPITATION.

1 Dr. Richard Reiss Sciences International, Inc. October 20, 2005 Environmental Safety of Active Pharmaceutical Ingredients.

Biodegradation of Emerging Contaminants by Pseudomonas butanorova Shervada Hall Houston Independent School District Empowerment College Preparatory High.

Waste Treatment Waste is a general problem in chemical plant operation especially in the developing country where the rules and regulations are very strict.

© 2015 Water Research Foundation. ALL RIGHTS RESERVED. © 2015 Water Research Foundation. ALL RIGHTS RESERVED. No part of this presentation may be copied,

Nitrification and the Removal of Micropollutants from Wastewater Arizona SpaceGrant Consortium Undergraduate Research Internship Program Symposium April.

COMmunity Programme of Research on Environmental Hormones and ENdocrine Disrupters.

Chemical Engineering Department 第二章表面活性剂 Chapter2 Surfactant 第四节乙氧基化与非离子表面活性剂生产技术.

The role of endocrine disrupting chemicals(EDCs) on estrogen receptors(ERs) using molecular docking Progress Report of DST Project Presented by Dr. Biswanath.

Conventional Sewage Treatment Plants:

Application of AOPs for the removal of nonylphenol and short-chain nonylphenol ethoxylates from water and wastewater effluent Klontza E.E.1,*, Xekoukoulotakis.

Yvonne Anna Minar, Barbara Milz and Bernd Spangenberg*

Endocrine Disruptors in Wastewater

Ozonation of estrogenic chemicals in biologically treated sewage

Analysis of Pharmaceuticals & Personal Care Products

Preliminary Treatment Removing Large Solids Material by Raking & Screening REMOVES 1.

Results and Discussion

Lecture (8): liquid wastes treatment (primary, vital, advanced).

ENDOCRINE DISRUPTING CHEMICALS and ENDOCRINE POTENCY of WASTEWATER measured by IN VIVO and IN VITRO ASSAYS K. Ole Kusk1, Hanne Frederiksen2,

FIG. 1. Comparison of luciferase signal in ZFL, ZELH, ZELH-zfERα, ZELH-zfERβ1, and ZELH-zfERβ2 cells either left untreated (DMSO control) or exposed to.

DETERMINATION OF ESTROGENIC STEROIDS IN SEWAGE SLUDGE SAMPLES FROM EIGHT DOMESTIC WASTEWATER TREATMENT PLANTS Jana Petre, Toma Galaon, Vasile Ion Iancu,

Checklist for Site Reconnaissance (Sabatini et al_2002)

Anaerobic Ammonium Oxidation (Anammox) for Nitrogen Removal

Using ultrasonic liquid extraction for estrogens analysis in sludge by HPLC with fluorescence detection Vitória Lourosa, Diana Limab, Jorge Leitãoc, Valdemar.

Presented By: Natasha Sinha

Photo-oxidation Process of Endocrine Disrupting Compounds in Water

EQS 17β-estradiol 17a-ethinyl-estradiol 0.4 ng/L 35 pg/L

Estrogenic Steroids Overall Organization of the Topic

Presentation transcript:

A Study of Endocrine Disrupting Compounds in Wastewater Arthur Ho Spacegrant Mentor: Dr. Bob Arnold Chemical and Environmental Engineering University of Arizona

Effects in Animals Kidd et al., 2007

USGS Surface Water Reconnaissance (Kolpin et al., 2002) 139 Sites 95 Compounds Comparison of 4 Compounds: Compound (Use and Effect)C MAX (µg/L)Days to Med Dose Caffeine (stimulant) x 10 3 (Excedrin Migraine) Ibuprofen(anti-inflammatory) (Motrin) 17α-ethinyl estradiol (oral contraceptive) (Alesse-21) 17β-estradiol (vertebrate hormone) (Phoenix)5.3 x 10 3 (Activella) 4-nonylphenol (surfactant metabolite) 40 (Tucson)4.6 x as estrogenic E 2, EE 2

Examples of Estrogenic Compounds ChemicalStructure Molecular Weight Log K ow Relative Estrogenic Activity (YES bioassay) 17  -estradiol (E 2 )  -ethinyl estradiol (EE 2 ) Estrone (E 1 ) Estriol (E 3 ) Nonylphenol (NP) ×10 -4 Octylphenol (OP) ×10 -4 Bisphenol A (BPA) ×10 -5

Estrogenic Activity at JWPCP Effluent: 0.12 mols EE2 equivalents/day Dewatered Cake: 0.26 mols EE 2 equivalents /day Influent: 1.66 mols EE 2 equivalents/day Removal of estrogenic activity through the whole treatment plant = [1.656 – ( )] / = 77%

Cytoplasm ERREE = Estrogen = Receptor Yeast Estrogen/ Androgen Screen R hER/hAR  -gal CPRG yellow CPR red R E lac-z

Yeast Estrogen Screen (YES) Assay

Cytoplasm ERREE = Estrogen = Receptor Yeast Estrogen/ Androgen Screen R hER/hAR  -gal CPRG yellow CPR red R E lac-z

Agonism Agonism Anti-estrogens: Mechanism of Action Antagonism Antagonism RE R A R E R A  -gal No protein production

Tamoxifen is an Anti-estrogen Known anti-estrogens: (hydroxy) tamoxifen, chlorinated hormones, Known anti-androgens: 4-,4’-DDE, flutamide

Methods Obtaining Wastewater samples Obtaining Wastewater samples Extraction of Organics Extraction of Organics Preparation of organics Preparation of organics Yeast Estrogen Assay Yeast Estrogen Assay

RESULTS RAW SLUDGE SOLID EXTRACTS ARE HIGHLY ESTROGENIC RAW SLUDGE LIQUID EXTRACTS ARE ANTI- ESTROGENIC

RESULTS DIGESTED SLUDGE SOLIDS LOST ESTROGENIC ACTIVITY DIGESTED SLUDGE LIQUIDS HIGHLY ANTI- ESTROGENIC

Acknowledgements Dave Newman & Sondra Teske Dave Newman & Sondra Teske Susan Brew Susan Brew Matt Giardiana and AzSGC Staff Matt Giardiana and AzSGC Staff Bingfeng Dong Bingfeng Dong