CENTRIFUGES AND GROWTH FACTORS

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CENTRIFUGES AND GROWTH FACTORS Method: 6 samples of blood; Centrifuge (Silfradent protocol); Histomorphometrical analysis of growth factors: TGF-1 e VEGF-A in Buffy Coat: 1) Buffy Coat, 2) Red-buffy coat Interface e 3) Red Blood Cells 1, 2, 3; Quantitative analysis of TGF-1 e VEGF-A in blood serum; - Histomorphometrical analysis of CD34+ cells in Red-Buffy Coat Interface.

MEDIFUGE- SILFRADENT CONTROL CENTRIFUGE SERUM BUFFY COAT RED BLOOD CELLS Buffy Coat Red- Buffy Coat Interface Red Blood 1 Red Blood 2 Red Blood 3

VEGF-A serum * * P < 0,05 vs control centrifuge VEGF-A serum concentration is lower in the samples treated with Medifuge-Silfradent compared with control centrifuge.

VEGF-A expression * P < 0,05 vs control centrifuge * The histomorphometrical analysis showed a greater expression of VEGF-A in the samples treated with Medifuge-Silfradent compared with control centrifuge.

VEGF-A Buffy Coat CONTROL MEDIFUGE * Histomorphometrical analysis (IOD) of VEGF-A showed a greater expression of this growth factor in the Buffy Coat of samples treated with Medifuge-Silfradent compared with control centrifuge. MEDIFUGE * * P < 0,05 vs control centrifuge

Red – Buffy Coat Interface VEGF-A Red – Buffy Coat Interface CONTROL Histomorphometrical analysis (IOD) of VEGF-A showed a greater expression of this growth factor in the Buffy Coat of samples treated with Medifuge-Silfradent compared with control centrifuge. MEDIFUGE * P< 0,05 vs control centrifuge *

VEGF-A Red Blood 1 CONTROL MEDIFUGE * Histomorphometrical analysis (IOD) of VEGF-A showed a greater expression of this growth factor in the Red Blood 1 of samples treated with Medifuge-Silfradent compared with control centrifuge. MEDIFUGE * P< 0,05 vs control centrifuge *

VEGF-A Red Blood 2 CONTROL MEDIFUGE * Histomorphometrical analysis (IOD) of VEGF-A showed a greater expression of this growth factor in the Red Blood 2 of samples treated with Medifuge-Silfradent compared with control centrifuge. MEDIFUGE * * P< 0,05 vs control centrifuge

VEGF-A Red Blood 3 CONTROL MEDIFUGE * Histomorphometrical analysis (IOD) of VEGF-A showed a greater expression of this growth factor in the Red Blood 3 of samples treated with Medifuge-Silfradent with control centrifuge. MEDIFUGE * * P< 0,05 vs control centrifuge

TGF-β1 Serum * * P< 0,05 vs control centrifuge TGF-β1 serum concentration is lower in samples treated with Medifuge-Silfradent compared with control centrifuge.

TGF-β1 expression * * * * * * P < 0,05 vs control centrifuge The histomorphometrical analysis showed a greater expression of TGF-β1 in samples treated with Medifuge-Silfradent compared with control centrifuge.

TGF-β1 Buffy Coat CONTROL MEDIFUGE * Histomorphometrical analysis (IOD) of TGF-β1 showed a greater expression of this growth factor in the Buffy Coat of samples treated with Medifuge-Silfradent compared with control centrifuge. MEDIFUGE * P < 0,05 vs control centrifuge *

Red – Buffy Coat Interface TGF-β1 Red – Buffy Coat Interface CONTROL Histomorphometrical analysis (IOD) of TGF-β1 showed a greater expression of this growth factor in the Red-Buffy Coat of samples treated with Medifuge-Silfradent compared with control centrifuge. MEDIFUGE * * P < 0,05 vs control centrifuge

TGF-β1 Red Blood 1 CONTROL MEDIFUGE * Histomorphometrical analysis (IOD) of TGF-β1 showed a greater expression of this growth factor in the Red Blood 1 of samples treated with Medifuge-Silfradent compared with control centrifuge. MEDIFUGE * * P < 0,05 vs control centrifuge

TGF-β1 Red Blood 2 CONTROL MEDIFUGE * Histomorphometrical analysis (IOD) of TGF-β1 showed a greater expression of this growth factor in the Red Blood 2 of samples treated with Medifuge-Silfradent compared with control centrifuge. MEDIFUGE * * P < 0,05 vs control centrifuge

TGF-β1 Red Blood 3 CONTROL MEDIFUGE * Histomorphometrical analysis (IOD) of TGF-β1 showed a greater expression of this growth factor in the Red Blood 3 of samples treated with Medifuge-Silfradent compared with control centrifuge. MEDIFUGE * * P < 0,05 vs control centrifuge

VEGF-A and TGF-β1 results The histomorphometric analysis (IOD) of blood growth factors TGF-1 and VEGF-A showed a greater expression of these growth factors in the samples treated with Medifuge-Silfradent compared with control centrifuge. Enzymatic analysis of VEGF-A e TGF-1 has confirmed our previous results showing a significant difference of both growth factors serum concentration: in particular, we found a lower serum concentration of both growth factors in the samples treated with Medifuge-Silfradent compared with control centrifuge. Bar 10 μm

Red- Buffy Coat Interface CD 34 Stem cells Red- Buffy Coat Interface CONTROL MEDIFUGE - The immunohistochemical analysis of the Red-Buffy Coat Interface showed a greater number of white cells in samples treated with Medifuge-Silfradent centrifuge with control centrifuge, as suggest by our previous analysis. - The immunohistochemical analysis of cells CD34+ expressed in the Red-Buffy Coat Interface showed a number of cells CD34+ four time greater in the samples trated with Medifuge-Silfradent centrifuge compared with control centrifuge.