© Mark E. Damon - All Rights Reserved

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© Mark E. Damon - All Rights Reserved Directions: Scroll through the presentation and enter the answers (which are really the questions) and the questions (which are really the answers). Enter in the categories on the main game boards. As you play the game, click on the TEXT DOLLAR AMOUNT that the contestant calls, not the surrounding box. When they have given a question, click again anywhere on the screen to see the correct question. Keep track of which questions have already been picked by printing out the game board screen and checking off as you go. Click on the “Game” box to return to the main scoreboard. Enter the score into the black box on each players podium. Continue until all clues are given. When finished, DO NOT save the game. This will overwrite the program with the scores and data you enter. You MAY save it as a different name, but keep this file untouched!

© Mark E. Damon - All Rights Reserved Round 1Round 2 Final Jeopardy TEAM 1 TEAM 2 TEAM 3

© Mark E. Damon - All Rights Reserved PCR Gels and other stuff… Recombi nant DNA Restricti on Maps Stem Cells Cloning & Misc Trivia $100 $200 $300 $400 $500 Final Jeopardy Scores

© Mark E. Damon - All Rights Reserved $100 This is abbreviated PCR

© Mark E. Damon - All Rights Reserved $100 What is Polymerase Chain Reaction Scores

© Mark E. Damon - All Rights Reserved $200 Define Polymerase Chain Reaction (what does it do?)

© Mark E. Damon - All Rights Reserved $200 Uses enzymes and heat to amplify small amounts of DNA Scores

© Mark E. Damon - All Rights Reserved $300 Give three ingredients for PCR and their purpose

© Mark E. Damon - All Rights Reserved $300 DNA Primers Taq Polymerase dNTPs Buffer Thermal Cycler DNA Primers Taq Polymerase dNTPs Buffer Thermal Cycler Scores

© Mark E. Damon - All Rights Reserved $400 What are primers (include what end they are complementary to)?

© Mark E. Damon - All Rights Reserved $400 Short pieces of DNA that are complementary to the 3’ ends of our target DNA Scores

© Mark E. Damon - All Rights Reserved $500 List the three stages of a PCR cycle. Include: 1.Approx. Temp 2. Name of the Stage 3.What happens during the stage List the three stages of a PCR cycle. Include: 1.Approx. Temp 2. Name of the Stage 3.What happens during the stage

© Mark E. Damon - All Rights Reserved $500 Denature (95C) – strands come apart Anneal (50C) – primers attach Extension (70C) – taq pol. Extends DNA Denature (95C) – strands come apart Anneal (50C) – primers attach Extension (70C) – taq pol. Extends DNA Scores

© Mark E. Damon - All Rights Reserved $100 What charge is DNA?

© Mark E. Damon - All Rights Reserved $100 Negative Scores

© Mark E. Damon - All Rights Reserved $200 Finish this sentence: The ________ the DNA, the ________ it will travel

© Mark E. Damon - All Rights Reserved $200 Larger ; Slower Scores

© Mark E. Damon - All Rights Reserved $300 List three ingredients of electrophoresis and describe their purpose

© Mark E. Damon - All Rights Reserved $300 Agarose Gel Electric Current Buffer DNA Dye Chamber Agarose Gel Electric Current Buffer DNA Dye Chamber Scores

© Mark E. Damon - All Rights Reserved

$400 In gel electrophoresis, we often use ethidium bromide as our dye. How does ethidium bromide work?

© Mark E. Damon - All Rights Reserved $400 It is a intercalating agent – it inserts itself in the DNA and it shines under a UV light – we can see where the DNA is Scores

© Mark E. Damon - All Rights Reserved $500 In DNA sequencing, we use dideoxynucleotides to stop the replication of the DNA. What is it about these molecules that stops replication (hint: I mentioned it very briefly)

© Mark E. Damon - All Rights Reserved $500 They are missing a 3’ -OH group Scores

© Mark E. Damon - All Rights Reserved $100 This is the term for DNA from different sources that has been combined into one new piece

© Mark E. Damon - All Rights Reserved $100 Recombinant DNA Scores

© Mark E. Damon - All Rights Reserved $200 What are restriction enzymes?

© Mark E. Damon - All Rights Reserved $200 Enzymes that cut DNA at specific sequences Scores

© Mark E. Damon - All Rights Reserved $300 If I treat two pieces of DNA with EcoRI, will I be able to recombine them? Why?

© Mark E. Damon - All Rights Reserved $300 Yes ; DNA treated with the same restriction enzymes produces complementary sticky ends Scores

© Mark E. Damon - All Rights Reserved $400 Explain the process of using recombinant DNA technology to manufacture insulin

© Mark E. Damon - All Rights Reserved $400 Scores

© Mark E. Damon - All Rights Reserved $500 What are the three components of a plasmid. List and describe

© Mark E. Damon - All Rights Reserved $500 1.Replicator 2.Selectable Marker 3.Cloning Site 1.Replicator 2.Selectable Marker 3.Cloning Site Scores

© Mark E. Damon - All Rights Reserved $100 What would result if we cut with EcoR1?

© Mark E. Damon - All Rights Reserved $100 One, 15.4 kb fragment Scores

© Mark E. Damon - All Rights Reserved $200 What would result if you cut with HindIII?

© Mark E. Damon - All Rights Reserved $200 Two fragments: 7.5 & 7.9 kb Scores

© Mark E. Damon - All Rights Reserved $300 What would result if you cut with ClaI?

© Mark E. Damon - All Rights Reserved $300 2 Fragments; 3.6 & 11.8 kb Scores

© Mark E. Damon - All Rights Reserved $400 What would result if you cut with Bg/II?

© Mark E. Damon - All Rights Reserved $400 3 fragments:.25, 4.6, & kb Scores

© Mark E. Damon - All Rights Reserved $500 What would result if you treated with EcoRV?

© Mark E. Damon - All Rights Reserved $500 6 Fragments:.65,.54, 3.1,.81, 2.4, 7.9 Scores

© Mark E. Damon - All Rights Reserved $100 What is differentiation?

© Mark E. Damon - All Rights Reserved $100 The process of a cell becoming specialized Scores

© Mark E. Damon - All Rights Reserved $200 List two differences between adult and embryonic stem cells

© Mark E. Damon - All Rights Reserved $200 Embryonic: from embryos ; can become any cell type ; made through cloning ; most potential Adult: from adults, infants, kids ; multipotent ; limited cells types ; found in limited locations Embryonic: from embryos ; can become any cell type ; made through cloning ; most potential Adult: from adults, infants, kids ; multipotent ; limited cells types ; found in limited locations Scores

© Mark E. Damon - All Rights Reserved $300 List and describe the three levels of cell potency

© Mark E. Damon - All Rights Reserved $300 Totipotent ; Pluripotent ; Multipotent Scores

© Mark E. Damon - All Rights Reserved $400 What are iPS cells?

© Mark E. Damon - All Rights Reserved $400 Induced Pluripotent Stem Cells: re-engineered stem cells that act like ESC - made through inserting either 4 genes or 4 gene products Scores

© Mark E. Damon - All Rights Reserved $500 What is asymmetric division?

© Mark E. Damon - All Rights Reserved $500 Stem Cell Self-Renewal – stem cells divide and one goes on to differentiate. The other remains a stem cell Scores

© Mark E. Damon - All Rights Reserved $100 What is cloning?

© Mark E. Damon - All Rights Reserved $100 The process of creating a genetically identical organism through nonsexual means Scores

© Mark E. Damon - All Rights Reserved $200 What is epigenetics?

© Mark E. Damon - All Rights Reserved $200 Gene expression caused by mechanisms other than DNA sequence Scores

© Mark E. Damon - All Rights Reserved $300 According to Wikipedia, what percentage of cab drivers in NYC are foreign-born

© Mark E. Damon - All Rights Reserved $ Percent Scores

© Mark E. Damon - All Rights Reserved $400 How long does it take the average person to fall asleep?

© Mark E. Damon - All Rights Reserved $400 7 minutes Scores

© Mark E. Damon - All Rights Reserved $500 What is acetylation and methylation?

© Mark E. Damon - All Rights Reserved $500 Mechanisms of Epigentics: Acetylation = turns on the gene Methylation = turns off the gene Mechanisms of Epigentics: Acetylation = turns on the gene Methylation = turns off the gene Scores

© Mark E. Damon - All Rights Reserved Scores Cloning Final Jeopary Question

© Mark E. Damon - All Rights Reserved As you are walking down the street, you hear someone mention that “a clone is an exact copy of another organism in every way including DNA, appearance, and personality.” You know this is not correct! Give four reasons for why clones are not exact replicas.

© Mark E. Damon - All Rights Reserved Epigenetics (methylation & acetylation) mtDNA X-inactivation Nature vs. Nurture (different environment / experiences) Epigenetics (methylation & acetylation) mtDNA X-inactivation Nature vs. Nurture (different environment / experiences) Scores