Hansen Online figure 1 GAPDH LANA 10 20 30 40 Skin P1Skin P2Skin P3 KS 330 KS P2 KS 248KS 606 KS 602 Threshold Cycle SkinKaposi sarcoma A B SkinKSSkinKSSkinKS.

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Presentation transcript:

Hansen Online figure 1 GAPDH LANA Skin P1Skin P2Skin P3 KS 330 KS P2 KS 248KS 606 KS 602 Threshold Cycle SkinKaposi sarcoma A B SkinKSSkinKSSkinKS

KS biopsy Skin C Hansen Online figure 1 Cont.

A Hansen Online figure 2 B K12-6-5pK12-11 * *** ** microRNA Delta Ct ** pSIN Individual lentivirus Cluster C ng Cluster K12-1K12-3K12-4-3pK12-5K12-6-3pK12-7K12-8K12-9K12-11 Relative RLU:PRO normalized to 0ng Cluster miRNA Reporter Construct 0ng Cluster 250ng Cluster 500ng Cluster * * ** * * * * * * *

D K12-6-3P MAF-3723bp UGCUUUUAACAAAAAAGCAUCU :I:IIIIIIIIII GAGUUGUCGGGCUUUUGGUAGU 5’ 3’ 5’ GUCUAGCUGGGCCUGAUGUCAA I:I:::IIIIIIII CGCUUGCCUUUUCACUAAAGUU MAF-2738bp K12-2 5’ 3’ 5’ CCGUACCUAAUCCACGACGACC III:IIIIIIIIIII AGGUUGUGUUUGGUGCUGCUAA MAF-3425bp K12-6-5p 5’ 3’ 5’ AGCCUGUGUCCGAUUCGUAAUU :I::IIIIIIII GUUGUGUAGAAUUAAGCAUUUU MAF-3850bp K ’ 3’ 5’ GAGUUGUCGGGCUUUUGGUAGU ::IIIII:IIII UGUACAUUAAAAAAAAUCAUCA MAF-4350bp K12-6-3p 5’ 3’ 5’ Hansen Online figure 2 Cont. K12-6-3P MAF-2638bp CUGAUCAGGAGCAAAGCCAUCC IIIIIIIII:IIIIII GAGUUGUCGGGCUUUUGGUAGU 5’ 3’ 5’ K12-1 MAF-2019bp 5’3’ 5’ UGCAGAACUGGAUUUUCUGUAAC :II::III:IIIIII CGAAUGUGGGUCAAAGGACAUUA AGC GGAUCUCAUGACGCCAAAUCGA ::IIIIIIIIIII AAAGAGUUAGGUCUGUUUAGCU MAF-1566bp K12-4-5p 5’ 3’5’ 3’ MAF-582bp CUCACUCCUUCUCAACCCAUCU IIII:::IIIIIIII GAGUUGUCGGGCUUUUGGUAGU 5’ 3’5’ 3’ K12-6-3p AGC GUAUUGAAAACCAAAGUAUUAA :IIIIII:IIIII AGCCUGUGUCCGAUUCGUAAUU MAF-1748bp 5’ 3’5’ 3’ K12-11 GCG

E pSIN K12-1K12-2K12-4K12-5K12-6K12-7K12-8 K12-10K12-11 K12-9 Relative MAF mRNA levels * * Hansen Online figure 2 Cont.

Cluster Relative MAF mRNA levels pCDNA ABC Hansen Online figure 3 * ** Cluster NTC Cluster i11 Cluster i6 Relative MAF mRNA levels pCDNA NTC pCDNA i11 pCDNA i6 Relative MAF mRNA levels

A GenesiMAFKLECClusterTotal NRCAM TFEC SLC1A CYYR11102 PALMD1012 HDAC91102 OSTM11102 IFI APBB21012 HSPB81012 IGF2BP31012 CSF2RB1102 EVI51102 PTPRG1102 LOC TNIK1012 GULP11102 AKAP Hansen Online figure 4 siControl siMAF MAML2FLT1CXCR4CXCL12MAF Relative mRNA levels ** * * B

Hansen Online figure 5 LEC markersBEC markers LEC PROX1 MAF FLT4 PDPN LYVE ITGB3 FLT1 SLC1A1 NRCAM HDAC9 TFEC CXCR4 GULP1 CXCL12 Relative mRNA levels normalized to LEC A B