Nathan Reyna, Biology Jeff Matocha, Computer Science Ouachita Baptist University Kentuck-E. Derby.

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Presentation transcript:

Nathan Reyna, Biology Jeff Matocha, Computer Science Ouachita Baptist University Kentuck-E. Derby

Project Goals Active, hands-on approach to teaching basic biology and computer science concepts Active, hands-on approach to teaching basic biology and computer science concepts Encourage abstract thinking Encourage abstract thinking Keeps students/faculty engaged in a semester long project. Keeps students/faculty engaged in a semester long project. Have fun Have fun

Project Overview Goal Goal – To engineer a biological “wire” capable of propagating a chemical signal down its length Initial Signal

Harvard iGEM 2005 Orr Ashenberg Patrick Bradley Connie Cheng Chris Doucette Hing Eng Jennifer Gao Kang-Xing Jin Yin Li Thomas Noriega Daniel Popper Alexander Rush Yves Wang Doh!

Requirements Inducible Promoter Inducible Promoter Product/Signal that activates promoters in other cells Product/Signal that activates promoters in other cells – Secrete product outside of cell Cell to cell communication/Quorum sensing Cell to cell communication/Quorum sensing Detection Method Detection Method

Method Signal: acyl-homoserine lactones (AHL) used in bacterial quorum sensing Signal: acyl-homoserine lactones (AHL) used in bacterial quorum sensing – Lux system: 3OC6HSL – Las system: 3OC12HSL – Sender device Other promoters associated with cell to cell communication Other promoters associated with cell to cell communication – Most are “in planning” stage – LacI generator – LacI promoter

Harvard’s Conclusions Conclusions Conclusions – Degradation tags, RBS/promoter strength may need fine-tuning – Because of positive feedback, noise is amplified – Further experiments necessary

GFP production across plate GFP production across plate Change in pH gradient Change in pH gradient Vernier conductivity probe Vernier conductivity probe – Senses changes in pH – Connects to a computer – Currently use probes in Freshman Bio and Gen Chem Tracking the Horses

Data acquisition Data acquisition – interfacing with probes Data reporting Data reporting CS student projects

Computer Science Time

Not just a one heat race Reduce pathway bottlenecks Reduce pathway bottlenecks Diffusion rates/distance between cells Diffusion rates/distance between cells

Questions What promoter/signal pairs do we have? What promoter/signal pairs do we have? Are they secreted outside the cell? Are they secreted outside the cell?