Pyruvate Oxidation Pyruvate dehydrogenase complex.

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Presentation transcript:

Pyruvate Oxidation Pyruvate dehydrogenase complex

Aerobic Fate of Pyruvate: Overview

Mitochondria: Site of Oxidations

Pyruvate Oxidation to Acetyl-CoA

Pyruvate Dehydrogenase Complex l Complex enzyme – 60 polypeptides of 3 kinds l In mitocondrion matrix l Regulated – Inhibited by NADH and GTP – Stimulated by insulin

Pyruvate Dehydrogenase Complex l Three enzymes and 5 coenzymes Total MW: 4.5 x 10 6 ! Also present: regulatory protein kinase, phosphatase

Lipoic Acid: Acyl- and Redox Carrier

Thiamine Pyrophosphate (TPP)

Pyruvate Dehydrogenase Mechanism

PDH: The Overall Reaction l Acetyl-CoA enter Krebs cycle l NADH passes e – to O 2

 -Ketoglutarate Dehydrogenase Complex ∆Gº= –33.5 kJ/mol

 -Ketoglutarate Dehydrogenase Complex l Cofactors: FAD, NAD +, lipoate, TPP, CoASH l Mechanism  PDH complex