KS + Ima + Zvad % of positive cells (ANV+) Cells positive for SA-β-gal (%) Cells positive for SA-β-gal (%) % of positive cells (ANV+) Supplementary Figure 1 n = 8 A-B- C-D- KS + Ima + Zvad

K562 K562 + Ima K562 + Ima + Zvad Supplementary Figure 1E BD Drullion et al AC

Hsp60 LC3B KSKS shATG7 Ima BafA Hsp60 LC3B Hsp60 LC3B KS KS shATG7 HBSS - BafA1 06h 12h9h 12h 06h 12h9h 12h HBSS + BafA1 Supplementary Figure 2 A- B-

KSKS shATG7 Control Ima Supplementary Figure 2C

K562 sh ATG7 K562 sh ATG7 + Ima K562 sh ATG7 + Ima + Zvad A B C D Supplementary Figure 3A Drullion et al

Ima Tetra--++ sh casp3sh casp9 Casp3 Casp9 p21 p27 hsp60 Cleaved Casp3 % of positive cells (ANV+) Supplementary Figure 4 n = 3 A- B-

Cells positive for SA-  -Gal (%) Supplementary Figure 4 n = 3 C-

sh-scr sh-Bcn control sh-ATG ATG5 Hsp 60 Beclin 1 Hsp 60 Supplementary Figure 5 A- Ima Zvad Mean fluorescence intensity n = 3 *p<0.05 * *

% of positive cells (ANV+) n = 4 *p<0.05 * Ima Zvad B- Cells positive for SA-b-Gal (%) n = 4 *p<0.05 *** Ima Zvad C- * Supplementary Figure 5

KS KS sh-scr Control Ima +ZVad KS sh-Bcn KS sh-ATG5 Supplementary Figure 5 D-