Apoptosis and Mitochondrial Dysfunction in Human Chondrocytes Following Exposure to Lidocaine, Bupivacaine, and Ropivacaine by Valentina Grishko, Min Xu,

Slides:

Advertisements

Similar presentations

Early Operation on Patients with a Hip Fracture Improved the Ability to Return to Independent Living by Amer N. Al-Ani, Bodil Samuelsson, Jan Tidermark,

Advertisements

Periprosthetic Humeral Fractures After Shoulder Arthroplasty by Sanjay Kumar, John W. Sperling, George H. Haidukewych, and Robert H. Cofield J Bone Joint.

Economic Credentialing and Physician Performance Measures: They Know Who You Are by David A. Wong, and Laura L. Forese J Bone Joint Surg Am Volume 92(5):

Radiographic and Histologic Assessment of Calcium Sulfate in Experimental Animal Models and Clinical Use as a Resorbable Bone-Graft Substitute, a Bone-Graft.

Optimal Timing of Preoperative Radiation for Prophylaxis Against Heterotopic Ossification by Mustasim N. Rumi, Gurvinder S. Deol, Jason A. Bergandi, Kishor.

Distal Radial Fractures in Older Men by Carl M. Harper, Shannon K. Fitzpatrick, David Zurakowski, and Tamara D. Rozental J Bone Joint Surg Am Volume 96(21):

Long-Term Follow-up After Latissimus Dorsi Transfer for Irreparable Posterosuperior Rotator Cuff Tears by Hossam Mahmoud El-Azab, Olaf Rott, and Ulrich.

Avoiding Complications in the Treatment of Humeral Fractures by Jeffrey O. Anglen, Michael T. Archdeacon, Lisa K. Cannada, and Dolfi Herscovici J Bone.

Effectiveness of a Microvascular Surgery Training Curriculum for Orthopaedic Surgery Residents by Jia-Wei Kevin Ko, Alyssa Lorzano, and Adam J. Mirarchi.

Computer Navigation for Total Knee Arthroplasty Reduces Revision Rate for Patients Less Than Sixty-five Years of Age by Richard N. de Steiger, Yen-Liang.

Chondroblastoma of Bone in the Extremities by Hairong Xu, Dylan Nugent, Hector L. Monforte, Odion T. Binitie, Yi Ding, G. Douglas Letson, David Cheong,

A Meta-Analysis Comparing the Results of Cervical Disc Arthroplasty with Anterior Cervical Discectomy and Fusion (ACDF) for the Treatment of Symptomatic.

Detection of Orthopaedic Implants in Vivo by Enhanced- Sensitivity, Walk-Through Metal Detectors by Miguel A. Ramirez, Edward K. Rodriguez, David Zurakowski,

Anti-Apoptotic Effects of Caspase Inhibitors on Rat Intervertebral Disc Cells by Jong-Beom Park, In-Chul Park, Sung-Jin Park, Hyeon-Ok Jin, Jin-Kyung Lee,

Nitric Oxide and Prostaglandin E2 Production in Response to Ultra-High Molecular Weight Polyethylene Particles Depends on Osteoblast Maturation State by.

Scoliosis in Patients with Duchenne Muscular Dystrophy by Lori A. Karol J Bone Joint Surg Am Volume 89(suppl 1): February 1, 2007 ©2007 by The Journal.

Symposium - The Outcomes Movement in Orthopaedic Surgery: Where We Are and Where We Should Go by MARC F. SWIONTKOWSKI, JOSEPH A. BUCKWALTER, ROBERT B.

The Fellowship Match Process: The History and a Report of the Current Experience by Lisa K. Cannada, Scott J. Luhmann, Serena S. Hu, and Robert H. Quinn.

Venous Thromboembolic Disease After Total Hip and Knee Arthroplasty by Steven B. Haas, Robert L. Barrack, Geoffrey Westrich, and Paul F. Lachiewicz J Bone.

Relationship Between Rates and Outcomes of Operative Treatment for Lumbar Disc Herniation and Spinal Stenosis by ROBERT B. KELLER, STEVEN J. ATLAS, DAVID.

Comparison of Ropivacaine and Bupivacaine Toxicity in Human Articular Chondrocytes by Samantha L. Piper, and Hubert T. Kim J Bone Joint Surg Am Volume.

Characteristics of Clinical Shoulder Research Over the Last Decade: A Review of Shoulder Articles in The Journal of Bone & Joint Surgery from 2004 to 2014.

Allograft-Prosthesis Composite for Revision of Catastrophic Failure of Total Elbow Arthroplasty by Pierre Mansat, Robert A. Adams, and Bernard F. Morrey.

Alfred R. Shands, Jr., Lecture: Our Humanitarian Orthopaedic Opportunity by Augustus A. White J Bone Joint Surg Am Volume 84(3): March 1, 2002 ©2002.

A Randomized, Prospective Study of Operative and Non- Operative Treatment of Injuries of the Fibular Collateral Ligaments of the Ankle* by P. POVACZ, F.

Dislocation of Rotating Hinge Total Knee Prostheses by William G. Ward, David Haight, Paul Ritchie, Stan Gordon, and Jeffrey J. Eckardt J Bone Joint Surg.

A Rare Acromioclavicular Joint Injury in a Twelve-Year-Old Boy by John M. Kirkos, Kyriakos A. Papavasiliou, Ioannis K. Sarris, and George A. Kapetanos.

An MBA: The Utility and Effect on Physicians' Careers by Selene G. Parekh, and Bikramjit Singh J Bone Joint Surg Am Volume 89(2): February 1, 2007.

Level of Evidence in Orthopaedic Journals by William T. Obremskey, Nick Pappas, Emad Attallah-Wasif, Paul Tornetta, and Mohit Bhandari J Bone Joint Surg.

N-Acetylcysteine Inhibits Post-Impact Chondrocyte Death in Osteochondral Explants by James A. Martin, Daniel McCabe, Morgan Walter, Joseph A. Buckwalter,

The Financial Impact of Orthopaedic Fellowship Training by Trevor Gaskill, Chad Cook, James Nunley, and R. Chad Mather J Bone Joint Surg Am Volume 91(7):

Association Between Decreased Bone Mineral Density and Severity of Distal Radial Fractures by Robert A.E. Clayton, Mark S. Gaston, Stuart H. Ralston, Charles.

Demographics of Traumatic Amputations in Children by Randall T. Loder J Bone Joint Surg Am Volume 86(5): May 1, 2004 ©2004 by The Journal of Bone.

Natural History of Early Onset Scoliosis by Pedro Fernandes, and Stuart L. Weinstein J Bone Joint Surg Am Volume 89(suppl 1):21-33 February 1, 2007 ©2007.

Nicotine Delays Tendon-to-Bone Healing in a Rat Shoulder Model by L.M. Galatz, M.J. Silva, S.Y. Rothermich, M.A. Zaegel, N. Havlioglu, and S. Thomopoulos.

An Unusual Cause of Low-Back Pain in Children by Glenn Lipton, Eric Riddle, Leslie Grissom, Temesgen Fitru, Harold Marks, and S. Jay Kumar J Bone Joint.

Recombinant Human Bone Morphogenetic Protein-2 Accelerates Healing in a Rabbit Ulnar Osteotomy Model by M. L. Bouxsein, T. J. Turek, C. A. Blake, D. D’Augusta,

The Trends in Treatment of Femoral Neck Fractures in the Medicare Population from 1991 to 2008 by Benjamin J. Miller, Xin Lu, and Peter Cram J Bone Joint.

Single-Leg-Stance Radiographs in the Diagnosis of Pelvic Instability by Jodi Siegel, David C. Templeman, and Paul Tornetta J Bone Joint Surg Am Volume.

Multipotential Differentiation of Human Anulus Fibrosus Cells by Gang Feng, Xinlin Yang, Hulan Shang, Ian W. Marks, Francis H. Shen, Adam Katz, Vincent.

Reconstruction of Segmental Bone Defects Due to Chronic Osteomyelitis with Use of an External Fixator and an Intramedullary Nail by Levent Eralp, Mehmet.

Sustained Spinal Cord Compression by Gregory D. Carlson, Carey D. Gorden, Shigenobu Nakazawa, Eiji Wada, Jeremy S. Smith, and Joseph C. LaManna J Bone.

Patellar Resurfacing in Primary Total Knee Replacement by R.W.D. Pilling, E. Moulder, V. Allgar, J. Messner, Z. Sun, and A. Mohsen J Bone Joint Surg Am.

Ilizarov Hip Reconstruction for the Late Sequelae of Infantile Hip Infection by S. Robert Rozbruch, Dror Paley, Anil Bhave, and John E. Herzenberg J Bone.

Prolonged Storage Effects on the Articular Cartilage of Fresh Human Osteochondral Allografts by Seth K. Williams, David Amiel, Scott T. Ball, R. Todd Allen,

Management of Capsular Deficiency of the Shoulder by Jon J.P. Warner, Andreas A. Venegas, Janne T. Lehtinen, and John J. Macy J Bone Joint Surg Am Volume.

Prognostic Indicators for Recurrent Symptoms After a Single Corticosteroid Injection for Carpal Tunnel Syndrome by Philip E. Blazar, W. Emerson Floyd,

Reliability and Reproducibility of Dens Fracture Classification with Use of Plain Radiography and Reformatted Computer-Aided Tomography by Lance Barker,

Variability in Spine Surgery Procedures Performed During Orthopaedic and Neurological Surgery Residency Training by Alan H. Daniels, Christopher P. Ames,

Metal Ion Levels After Metal-on-Metal Total Hip Arthroplasty by C.A. Engh, S.J. MacDonald, S. Sritulanondha, A. Korczak, D. Naudie, and C. Engh J Bone.

Fistula between the Hip and the Sigmoid Colon after Total Hip Arthroplasty. A Case Report* by JARED S. LEVIN, AGUSTIN A. RODRIGUEZ, and KEVIN LUONG J Bone.

Lower-Extremity Peripheral Nerve Blocks in the Perioperative Pain Management of Orthopaedic Patients by Benjamin E. Stein, Umasuthan Srikumaran, Eric W.

Microcomputed Tomography Characterization of Shoulder Osseous Deformity After Brachial Plexus Birth Palsy: A Rat Model Study by Zhongyu Li, Jonathan Barnwell,

The Clamshell Osteotomy: A New Technique to Correct Complex Diaphyseal Malunions by George V. Russell, Matt L. Graves, Michael T. Archdeacon, David P.

Ultrasound Measurements of Torsions in the Tibia and Femur by David Hudson, Todd Royer, and James Richards J Bone Joint Surg Am Volume 88(1): January.

Periacetabular Osteotomy: the Bernese Experience by Klaus A Siebenrock, Michael Leunig, and Reinhold Ganz J Bone Joint Surg Am Volume 83(3): March.

Temporary Internal Distraction as an Aid to Correction of Severe Scoliosis by Jacob M. Buchowski, David L. Skaggs, and Paul D. Sponseller JBJS Essent Surg.

Transtibial Amputees from the Vietnam War by Paul J. Dougherty J Bone Joint Surg Am Volume 83(3): March 1, 2001 ©2001 by The Journal of Bone and.

Systemic Vibrio Infection Presenting as Necrotizing Fasciitis and Sepsis by Yao-Hung Tsai, Robert Wen-Wei Hsu, Kuo-Chin Huang, Chih-Hung Chen, Chin- Chang.

The Need for Increased Access to the U.S. Health-Care System by Jesse E. Bible, Richard S. Lee, and Gary E. Friedlaender J Bone Joint Surg Am Volume 91(2):

Complications Observed Following Labral or Rotator Cuff Repair with Use of Poly-L-Lactic Acid Implants by L. Pearce McCarty, Daniel D. Buss, Milton W.

An AOA Critical Issue: Aging of the North American Population: New Challenges for Orthopaedics* by Joseph A. Buckwalter, James D. Heckman, and David P.

Time Trade-Off as a Measure of Health-Related Quality of Life: Long Bone Nonunions Have a Devastating Impact by Patrick C. Schottel, Daniel P. O’Connor,

Mitochondrial DNA damage is involved in apoptosis caused by pro-inflammatory cytokines in human OA chondrocytes J. Kim, M. Xu, R. Xo, A. Mates, G.L.

Ropivacaine- and bupivacaine-induced death of rabbit annulus fibrosus cells in vitro: involvement of the mitochondrial apoptotic pathway X.-Y. Cai, Y.

V. I. Grishko, Ph. D. , R. Ho, Ph. D. , G. L. Wilson, Ph. D. , A. W

Mitochondrial DNA damage is involved in apoptosis caused by pro-inflammatory cytokines in human OA chondrocytes J. Kim, M. Xu, R. Xo, A. Mates, G.L.

Fig. 3. Effects of Tec on IL-1β-induced apoptosis in chondrocytes.

CYP1A1 knockdown increases cell death.

Mitotic catastrophe symptoms caused by curcumin are followed by apoptosis. Mitotic catastrophe symptoms caused by curcumin are followed by apoptosis. A.

Presentation transcript:

Apoptosis and Mitochondrial Dysfunction in Human Chondrocytes Following Exposure to Lidocaine, Bupivacaine, and Ropivacaine by Valentina Grishko, Min Xu, Glenn Wilson, and Albert W. Pearsall J Bone Joint Surg Am Volume 92(3): March 1, 2010 ©2010 by The Journal of Bone and Joint Surgery, Inc.

Scatterplots of flow cytometry determinations of the numbers of necrotic, apoptotic, and viable cells twenty-four hours after a one-hour exposure to the following (from left to right, from top to bottom): saline solution, 2% lidocaine, 1% lidocaine, 0.5% li... Valentina Grishko et al. J Bone Joint Surg Am 2010;92: ©2010 by The Journal of Bone and Joint Surgery, Inc.

Viability of human chondrocytes as determined by flow cytometry analysis at twenty-four hours, seventy-two hours, and 120 hours after exposure to saline solution (C), 1% and 0.5% lidocaine (L) (top panel); 0.5% and 0.25% bupivacaine (B), and 0.5 and 0.2% ro... Valentina Grishko et al. J Bone Joint Surg Am 2010;92: ©2010 by The Journal of Bone and Joint Surgery, Inc.

Scatterplots of flow cytometry determinations of the numbers of necrotic, apoptotic, and viable cells 120 hours after a one-hour exposure to the following (from left to right, from top to bottom): saline solution, 1% lidocaine, 0.5% lidocaine, 0.5% bupivaca... Valentina Grishko et al. J Bone Joint Surg Am 2010;92: ©2010 by The Journal of Bone and Joint Surgery, Inc.

Apoptosis in human chondrocytes at 120 hours following a single one-hour exposure to local anesthetics. Valentina Grishko et al. J Bone Joint Surg Am 2010;92: ©2010 by The Journal of Bone and Joint Surgery, Inc.

Western blot analysis of procaspase 3 and 9 levels in human chondrocytes at 120 hours following exposure to 0.5% and 1% lidocaine (L0.5 and L1), 0.5% and 0.2% ropivacaine (R0.5 and R0.2), and 0.5% and 0.25% bupivacaine (B0.5 and B0.25). Valentina Grishko et al. J Bone Joint Surg Am 2010;92: ©2010 by The Journal of Bone and Joint Surgery, Inc.

Mitochondrial DNA damage induced by local anesthetics in human chondrocytes. Valentina Grishko et al. J Bone Joint Surg Am 2010;92: ©2010 by The Journal of Bone and Joint Surgery, Inc.

Effect of tetrodotoxin (TTX) on human chondrocyte mtDNA integrity. Valentina Grishko et al. J Bone Joint Surg Am 2010;92: ©2010 by The Journal of Bone and Joint Surgery, Inc.

Effect of high potassium (KCl) on mtDNA damage in human chondrocytes following local anesthetic exposure. Valentina Grishko et al. J Bone Joint Surg Am 2010;92: ©2010 by The Journal of Bone and Joint Surgery, Inc.

ATP levels (in pmol/1000 cells) in human chondrocytes following exposure to 0.5%, 1%, and 2% lidocaine (L), 0.5% and 0.25% bupivacaine (B), and 0.5% and 0.2% ropivacaine (R). Valentina Grishko et al. J Bone Joint Surg Am 2010;92: ©2010 by The Journal of Bone and Joint Surgery, Inc.

Western blot analysis of cytochrome-c oxidase subunit III (Sub III COX) levels in human chondrocytes following exposure to saline solution and to 2%, 1%, and 0.5% lidocaine (from left to right). Valentina Grishko et al. J Bone Joint Surg Am 2010;92: ©2010 by The Journal of Bone and Joint Surgery, Inc.