HPLC Pigment Analysis at HPL Crystal S. Thomas Laurie Van Heukelem VIIRS/MODIS Science Team Meeting May 14, 2008.

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Presentation transcript:

HPLC Pigment Analysis at HPL Crystal S. Thomas Laurie Van Heukelem VIIRS/MODIS Science Team Meeting May 14, 2008

Outline QA/QC QA/QC Progress report Progress report What I need from PIs What I need from PIs

Chl a calibration

Calculation Equation for Pigment Concentration Our QC measurements check every variable in our calculation equation

Examples of Daily QC Measurements Injector precision Injector precision Avg CV%=0.4%, WL=0.8%, CL=1.3% Avg CV%=0.4%, WL=0.8%, CL=1.3% Accuracy of chl a standard Accuracy of chl a standard Avg=-0.75%,WL=4.1%, CL=5.3% Avg=-0.75%,WL=4.1%, CL=5.3% Calibration of solvent delivery device Calibration of solvent delivery device Avg CV%=0.34%, WL=0.57%, CL=0.83% Avg CV%=0.34%, WL=0.57%, CL=0.83% Retention time variability (on average no more than 0.07% within a sequence) Retention time variability (on average no more than 0.07% within a sequence) Peak resolution documentation (Rs> 1.0) Peak resolution documentation (Rs> 1.0) Carryover (no more than 0.1%) Carryover (no more than 0.1%)

Analysis precision v. method precision Daily analysis precision: Replicate injections of sample extracts (min and max residence time) Daily analysis precision: Replicate injections of sample extracts (min and max residence time) Daily method precision: Duplicate filters Daily method precision: Duplicate filters Analysis precision (CV%) Method filter precision for HPL clients (CV%) TChl a0.4 (WL=1.6%,CL=2.2%) 4 (1-10) PPig1.8 (WL=4.7%,CL=6.2%) 7 (2-11)

Control Charts

Looking at QC Measurements in Context No analysis = ISTD measurements = chl a QC measurements

Progress Report This year we will analyze 3700 pigment samples for NASA This year we will analyze 3700 pigment samples for NASA So far: So far: Received 1205 samples Received 1205 samples Analyzed 575 samples Analyzed 575 samples Have 630 samples still in the queue Have 630 samples still in the queue Know of ~300 more samples to be shipped soon Know of ~300 more samples to be shipped soon

What I need from PIs submitting pigment samples Duplicate filters-at least 5% Duplicate filters-at least 5% Completed, correct sample information forms Completed, correct sample information forms Communication, please contact me! Communication, please contact me!