© Synthesis Of A New Amino Acid/Sulfur Dioxide Copolymer And Its Use In Aqueous Two-Phase Polymer Systems Ali, SA; Al-Muallem, HA; Wazeer, MIM JOHN WILEY SONS INC, JOURNAL OF POLYMER SCIENCE PART A-POLYMER CHEMISTRY; pp: ; Vol: 40 King Fahd University of Petroleum & Minerals Summary The amine salt N,N-diallyl-N-5-carbomethoxypentylammonium chloride was copolymerized with sulfur dioxide in dimethyl sulfoxide with ammonium persulfate or 2,2'-azobisisobutyronitrile and afforded a cationic polyelectrolyte (CPE) with a five-membered cyclic structure on the polymeric backbone. The CPE, upon acidic hydrolysis of the pendent ester groups, gave a corresponding cationic acid salt (CABS) having the equivalent of chloride salt of 6-N,N-diallylammoniohexanoic acid as the monomeric unit. The CAS was converted into an anionic polyelectrolyte (APE) and a polybetaine (PB), having the monomeric unit equivalent of sodium 6-N,N- diallylaminohexanoate and 6-N,N-diallylammoniohexanoate, by treatment with 2 and 1 equiv of base, respectively. The solution properties of APE were investigated by potentiometric and viscometric techniques. The basicity constant of the amine functionality in APE was apparent and as such followed the modified Henderson- Hasselbalch equation; the protonation of the APE became more and more difficult as the degree of protonation of the whole macromolecule increased. The compositions and phase diagrams of the aqueous two-phase systems of APE and poly(ethylene glycol) were studied. The PB was found to be insoluble in water, and this paves the way for the potential use of APE in aqueous two-phase polymer systems for protein purification and its removal and recycling by conversion into PB. (C) 2002 Wiley Periodicals, Inc. References: Copyright: King Fahd University of Petroleum & Minerals;

© *STANF RES I, 1983, CHEM EC HDB, P581 ALBERTSSON P, 1986, PARTITION CELL PARTI ALI MM, 2000, POLYMER, V41, P5591 ALI SA, 1997, POLYMER, V38, P3385 ALI SA, 1999, POLYMER, V40, P2439 ALI SA, 1999, POLYMER, V40, P6849 ALMUALLEM HA, 2002, POLYMER, V43, P1041 ANTON P, 1993, MAKROMOL CHEM, V194, P601 BARBUCCI R, 1981, MACROMOLECULES, V14, P1203 BARBUCCI R, 1983, MACROMOLECULES, V16, P456 BARBUCCI R, 1986, MACROMOLECULES, V19, P1856 BARBUCCI R, 1986, MACROMOLECULES, V19, P37 BUTLER GB, 1951, J AM CHEM SOC, V73, P895 BUTLER GB, 1982, ACCOUNTS CHEM RES, V15, P370 BUTLER GB, 1992, CYCLOPOLYMERIZATION FELTY WJ, 1978, J CHEM EDUC, V55, P576 FERRUTI P, 1984, ADV POLYM SCI, V58, P57 HACHEM F, 1996, ENZYME MICROB TECH, V19, P507 HAMAD EZ, 1996, BIOTECHNOL PROGR, V12, P173 HARADA S, 1966, MAKROMOL CHEM, V90, P177 HARADA S, 1967, MAKROMOL CHEM, V107, P64 HUGHES P, 1988, ENZYME MICROB TECHNO, V10, P115 KALADAS JJ, 1998, POLYM PREPR, V39, P619 KULA MR, 1982, ADV BIOCHEM ENG, V24, P73 LU M, 1997, J CHROMATOGR A, V766, P99 MCCORMICK CL, 1996, J POLYM SCI POL CHEM, V34, P911 MCCORMICK CL, 1997, POLYM PREPR, V38, P91 MCCORMICK CL, 1998, POLYM PREPR, V39, P617 MONROYSOTO VM, 1984, POLYMER, V25, P254 MUMICK PS, 1994, MACROMOLECULES, V27, P323 MUTHUKUMAR M, 1996, J CHEM PHYS, V104, P691 OTTENBRITE RM, 1980, IND ENG CHEM PROD RD, V19, P528 SALAMONE JC, 1977, POLYMER, V18, P1058 SALAMONE JC, 1978, POLYMER, V19, P1157 SALI A, 1994, NATURE, V369, P248 SCHULZ DN, 1986, POLYMER, V27, P1734 SRIVASTAVA D, 1996, MACROMOLECULES, V29, P2324 THOMAS DB, 1999, POLYM PREPR AM CHEM, V40, P275 THOMAS DB, 1999, POLYM PREPR, V40, P193 WIELEMA TA, 1987, EUR POLYM J, V23, P947 WIELEMA TA, 1990, EUR POLYM J, V26, P415 WOLYNES PG, 1995, SCIENCE, V267, P1619 For pre-prints please write to: Copyright: King Fahd University of Petroleum & Minerals;