Q1. What is the role of DNA polymerase I in chromosomal replication? A) Synthesizes most of the new DNA strands E) None of the above C)Joins Okazaki fragments together B)Replaces RNA primers with DNA D)Both B) and C) are correct

Q2. Primase: A) Creates 3’ ends to which DNA Pol III adds dNTPs E) None of the above C)Acts multiple times during synthesis of each lagging strand B)Acts one time during synthesis of each leading strand D)All of the above

Q3. The following 2 pieces of DNA are placed in a test tube, along with dNTPs and with DNA polymerase. 5’ ATGTCAAC 3’ 3’GACTACAGTTGACGTACG5’ What would be the final product(s) after DNA polymerization occurs? 5’GACATGTCAACACGTACG3’ 3’GACTACAGTTGACGTACG5’ 5’CTGATGTCAACTGCATGC3’ 3’GACTACAGTTGACGTACG5’ 5’CTGATGTCAAC 3’ 3’GACTACAGTTGACGTACG5’ 5’ ATGTCAACTGCATGC3’ 3’GACTACAGTTGACGTACG5’ A) E) None of the above C) B) D)D)

Q4. What is the minimum number of DNA pol III molecules needed by E. coli to replicate its chromosome? A) 1 E) > 20 C)4 B)2 D)8