2012 GEM Midwest Trial Results & New Releases M. Blanco USDA-ARS NCRPIS GEM - December 5, 2012- G ermplasm E nhancement of M aize.

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2012 GEM Midwest Trial Results & New Releases M. Blanco USDA-ARS NCRPIS GEM - December 5, G ermplasm E nhancement of M aize

Commercial Checks 2012 Estimated Maturity Rank (Earliest to Latest) Hybrid Midwest RM HC33xLH287~109 Pioneer 34R65109 MBS3633xMBS Pioneer 33F85114 LH200xLH262~118 Pioneer 31D58119

G ermplasm Enhancement of Maize GEMS-0234 AR17026:S1648-B-030 See GEM Releases, Page Tester: LH287 (5 Reps) Tester: MBS8814 (7 Reps) PedigreeEntry Yield B/A % MoistYMTWTSLRLEntry Yield B/A % MoistYMTWTSLRL AR17026:S1648-B Check Means CV F-value LSD (p=0.05) Rating for SLB and 95% free of Head Smut 1 st year data 1 to 9 scale (9 = most resistant)

G ermplasm Enhancement of Maize GEMS-0235 CUBA173:S0446-B Tester: AGR-nSS BT1 (5 Reps) Tester: MBS8814 (8 Reps) PedigreeEntry Yield B/A % MoistYMTWTSLRLEntry Yield B/A % MoistYMTWTSLRL CUBA173:S0446-B Check Means CV F-value LSD (p=0.05) See GEM Releases, Page 4

G ermplasm Enhancement of Maize GEMN-0236 DK212T:N11a10-B Tester: HC33 (5 Reps) Tester: MBS3633 (8 Reps) PedigreeEntry Yield B/A % MoistYMTWTSLRLEntry Yield B/A % MoistYMTWTSLRL DK212T:N11a10-B Check Means CV F-value LSD (p=0.05) Rating for SLB 1 st year data 1 to 9 scale (9 = most resistant) See GEM Releases, Page 5

G ermplasm Enhancement of Maize GEMS-0237 DKXL370:S08c17c-B Tester: LH287 (5 Reps) Tester: MBS8814 (8 Reps) PedigreeEntry Yield B/A % MoistYMTWTSLRLEntry Yield B/A % MoistYMTWTSLRL DKXL370:S08c17c-B Check Means CV F-value LSD (p=0.05) % protein See GEM Releases, Page 5

G ermplasm Enhancement of Maize GEMN-0238 NEI9004:N0803-B Tester: Beck's SS (5 Reps) Tester: HC33 (8 Reps) PedigreeEntry Yield B/A % MoistYMTWTSLRLEntry Yield B/A % MoistYMTWTSLRL NEI9004:N0803-B Check Means CV F-value LSD (p=0.05) Rating for armyworm/earworm in Puerto Rico 1 st year data 1 to 9 scale (9 = most resistant) See GEM Releases, Page 6

G ermplasm Enhancement of Maize GEMN-0239 NEI9004:N0803-B Tester: Beck's SS (5 Reps) Tester: HC33 (8 Reps) PedigreeEntry Yield B/A % MoistYMTWTSLRLEntry Yield B/A % MoistYMTWTSLRL NEI9004:N0803-B Check Means CV F-value LSD (p=0.05) Rating for SLB 1 st year data 1 to 9 scale (9 = most resistant) See GEM Releases, Page 6

G ermplasm Enhancement of Maize GEMS-0240 UR13085:S99g99u-B Tester: LH287 (5 Reps) Tester: MBS8814 (7 Reps) PedigreeEntry Yield B/A % MoistYMTWTSLRLEntry Yield B/A % MoistYMTWTSLRL UR13085:S99g99u-B Check Means CV F-value LSD (p=0.05) % protein See GEM Releases, Page 6

G ermplasm Enhancement of Maize GEMS-0241 UR13085:S99g99u-B Tester: LH287 (5 Reps) Tester: MBS8814 (7 Reps) PedigreeEntry Yield B/A % MoistYMTWTSLRLEntry Yield B/A % MoistYMTWTSLRL UR13085:S99g99u-B Check Means CV F-value LSD (p=0.05) % Oil 7.5 SLB Rating 1 st year data 1 to 9 scale (9 = most resistant) See GEM Releases, Page 6

G ermplasm Enhancement of Maize Top Cross Estimated Relative Maturity x LH287x MBS8814x HC33x MBS3633 Estimated GEM CodePedigreeRM GEMS-0234AR17026:S1648-B GEMS-0235CUBA173:S0446-B ?118-- GEMN-0236DK212T:N11a10-B GEMS-0237DKXL370:S08c17c-B GEMN-0238NEI9004:N0803-B GEMN-0239NEI9004:N0803-B GEMS-0240UR13085:S99g99u-B GEMS-0241UR13085:S99g99u-B See GEM Releases, Page 3

G ermplasm Enhancement of Maize Fusarium & Diplodia M. Mancl, Pioneer Hi-Bred International (Woodland, CA) B. Dolezal, Pioneer Hi-Bred International (Johnston, IA) 4 Lines All Years Fusarium GEMS-0032 GEMS-0062 GEMS-0068 GEMN-0094 GEMN-0098 GEMN-0135 GEMS-0162 GEMN-0164 Diplodia GEMS-0018 Above Average Resistance Fusarium GEMN-0165 GEMS-0166 GEMN-0169 GEMN-0172 GEMN-0173 GEMN-0212 GEMN Lines All Years

G ermplasm Enhancement of Maize Head Smut 43 Lines All Years GEMS-0180 GEMS-0181 GEMS-0182 GEMS-0183 GEMS-0184 GEMN-0187 GEMN-0191 Above Average Resistance M. Mancl, Pioneer Hi-Bred International (Woodland, CA)

G ermplasm Enhancement of Maize Southern Leaf Blight Resistance (SLB) Above Average Resistance GEMN-0213 GEMN Lines All Years J. Dodd, Professional Seed Research, Inc P. Balint-Kurti, USDA-ARS PSRU

G ermplasm Enhancement of Maize Breeding Cross Obs (Thailand) Commercial Thai Hybrid General appearance rating 9 Resistant to DM T. Welsh, Seed Asia (Downy Mildew Stress)

G ermplasm Enhancement of Maize Breeding Cross Obs (Thailand) SX1078:S(PHJ40) No Thai Germplasm General appearance rating 3 Moderately resistant to DM T. Welsh, Seed Asia (Downy Mildew Stress)

G ermplasm Enhancement of Maize Breeding Cross Obs (Thailand) (Thai/GEMN-0136)/41 25% Thailand General appearance rating 7 Resistant to DM T. Welsh, Seed Asia (Downy Mildew Stress)

G ermplasm Enhancement of Maize Global Disease Evaluations of GEM Mal de Rio Cuarto in Argentina (Fiji virus group); Argentina Maize Rough Dwarf (MRDV) also Fiji virus group; Spain and China Maize Wilt or Late Wilt Cephalosporium maydis; Spain Head Smut; China, Spain, U.S. NLB; Turkey, Thailand, Canada, U.S. Southern Rust; Brazil, Thailand, China, U.S. (host differentials issue) GLS; importance for S. Africa, China, U.S.