NYC/NJ Fish eDNA Project

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Presentation transcript:

NYC/NJ Fish eDNA Project Mapping fish with environmental DNA (eDNA) Monitoring fish distribution and abundance Detecting invasive, endangered species Potentially faster, cheaper, gentler than traditional methods Rockefeller University gggg Monmouth University Monmouth University and Rockefeller University Marine Research Initiative (MURU) RU Summer Science Research Program, RU Program for the Human Environment

City Fish: NYC Central Park eDNA survey Iman Nassef Alden Liang *sampling sites summer 2015

A dozen species in ¼ cup of water Analyze 6.33 ng DNA (roughly 60 mL H2O); 7 x 10^4 reads Detected 7 species of fish, also mammals, birds Fish Mammals, birds The Loch (Note lower scale) Number of reads Fish images NYS DEC

eDNA detected most species found by traditional methods, plus some not found vs. Electrofishing vs. Seining pumpkinseed Yellow perch banded killifish bluegill largemouth bass Green sunfish golden shiner brown bullhead 6 1 2 black crappie 2 5 NYSDEC Harlem Meer Surveys, 2008-2013 2013 Bioblitz, Harlem Meer, The Pool

eDNA detected a minnow usually overlooked Image NYS DEC Banded killifish (Fundulus diaphanus) Range: northeastern US Adult size: 10-13 cm Last reported in 2003 Central Park Bioblitz, location not given Not reported in 2013 Bioblitz, 2008-2013 electrofishing surveys

People, Partners, Sponsors MURU Marine Research Initiative A collaboration between: Jeanne Garbarino LABORATORY OF GENETICALLY ENCODED SMALL MOLECULES Zachary Charop-Powers Howard Rosenbaum