Incubators including Peltier KT 115 September 2012 STRICTLY CONFIDENTIAL - FOR COMPANY USE ONLY 1 Welcome BINDER Incubators and the introduction of the new BINDER KT 115 with Peltier technology NEW

Incubators including Peltier KT 115 September 2012 STRICTLY CONFIDENTIAL - FOR COMPANY USE ONLY 2 Contents to fulfill the objective  Introduction  Technical Basics  Portfolio  Positioning KT – KB  Competition  USPs  Product Launch

Incubators including Peltier KT 115 September 2012 STRICTLY CONFIDENTIAL - FOR COMPANY USE ONLY 3 1.2What is an Incubator for biological/medical applications?  WIKIPEDIA: “A device used to grow and maintain microbiological cultures or cell cultures”  The incubator maintains optimal growth conditions Temperature Humidity Other parameters, such as CO 2 and O 2

Incubators including Peltier KT 115 September 2012 STRICTLY CONFIDENTIAL - FOR COMPANY USE ONLY 4 1.3Temperature Different ideal temperatures for different organisms  Mammalian cells 37 °C  Bacteria  Escherichia coli (intestines)37 °C  Pseudomonas (ubiquitous)28 °C  Agrobacterium (soil)21 °C  Vibrio fischeri (sea)15 °C  Thermus aquaticus 74 °C  Yeast  Saccharomyces cerevisiae30 °C  Lab temperature °C

Incubators including Peltier KT 115 September 2012 STRICTLY CONFIDENTIAL - FOR COMPANY USE ONLY 5 1.3Temperature Different ideal temperatures for different organisms laboratory temperature

Incubators including Peltier KT 115 September 2012 STRICTLY CONFIDENTIAL - FOR COMPANY USE ONLY 6 1.4Incubators of different kind Cool or uncool  Standard incubators  Can only heat up  Typical temperature range Minimum: 5 K to 10 K above ambient room temperature Maximum: 60 °C to 100 °C  Typical application: bacterial incubation at 37 °C

Incubators including Peltier KT 115 September 2012 STRICTLY CONFIDENTIAL - FOR COMPANY USE ONLY 7 1.5Incubators of different kind Cool or uncool  Cooling incubators  Can heat up and cool down  Typical temperature range Minimum: -5 °C to 5 °C Maximum: 60 °C to 100 °C  Various applications Incubation with heat input, e.g. by internal shaker Incubation at high ambient temperatures Incubation at 30 °C or lower  Cooling incubators with light  Typical applications Plant growth Insect growth Photostability testing

Incubators including Peltier KT 115 September 2012 STRICTLY CONFIDENTIAL - FOR COMPANY USE ONLY 8 1.6Incubators of different kind CO 2 or O 2  CO 2 Incubators  Can only heat up  Provide CO 2 and humidity  Provide O 2 option  Typical application: mammalian cell culture at 37 °C

Incubators including Peltier KT 115 September 2012 STRICTLY CONFIDENTIAL - FOR COMPANY USE ONLY 9 1.7Who needs Incubators? How and where to find customers  Universities and Research Institutes  Microbiology  Genetics  Zoology  Biochemistry  Health Clinics, Hospitals  Diagnostics  Biomedicine  Medical research  Reproductive medicine  Regenerative medicine  Industry  Pharma  Biotech  Food & Beverage  Cosmetics  Contract research

Incubators including Peltier KT 115 September 2012 STRICTLY CONFIDENTIAL - FOR COMPANY USE ONLY Who needs Incubators? How and where to find customers  Areas  Basic research  Medical research  Cell Biology  Pharmacology  Drug discovery  Cell therapy  In vitro fertilization (IVF)  Genetic engineering  Applications  Molecular biology  Sterility testing  Cell production  Antibody production  High throughput screening (HTS)  Tissue engineering  Model plant growth  Germ identification

Incubators including Peltier KT 115 September 2012 STRICTLY CONFIDENTIAL - FOR COMPANY USE ONLY 11 Contents to fulfill the objective  Introduction  Technical Basics  Incubator Portfolio  Positioning KT – KB  Competition  USPs  Product Launch

Incubators including Peltier KT 115 September 2012 STRICTLY CONFIDENTIAL - FOR COMPANY USE ONLY BINDER Incubators Large portfolio  Standard incubators  BD, BF  CO 2 incubators  C, CB  Cooling incubators  KB  Cooling incubators with Peltier technology  KT  Cooling incubators with light  KBW, KBWF

Incubators including Peltier KT 115 September 2012 STRICTLY CONFIDENTIAL - FOR COMPANY USE ONLY BINDER Incubators Large size selection: 28 different chambers * KT 53 available end 2012

Incubators including Peltier KT 115 September 2012 STRICTLY CONFIDENTIAL - FOR COMPANY USE ONLY Common Features of BINDER Incubators APT.line – Advanced Preheating Technology  Forced convection  Fan-driven  Chamber is surrounded by an air jacket  Heat is distributed by a powerful fan  Air is pulled into the preheating chamber  Air is blown out horizontally through small gaps in the side walls from both sides  Rapid heat distribution  BF, KB, KT incubators

Incubators including Peltier KT 115 September 2012 STRICTLY CONFIDENTIAL - FOR COMPANY USE ONLY Common Features of BINDER Incubators Effective disinfection/sterilization  BINDER Incubators can heat up to at least 100 °C  Providing a convenient and effective disinfection  Important anti-contamination feature for microbiology  BINDER CO 2 Incubators go even higher  180 °C automatic sterilization cycle  Unique anti-contamination feature for serious cell culturing 180°C100°C

Incubators including Peltier KT 115 September 2012 STRICTLY CONFIDENTIAL - FOR COMPANY USE ONLY 16 Contents to fulfill the objective  Introduction  Technical Basics  Portfolio  Positioning KT – KB  Competition  USPs  Product Launch

Incubators including Peltier KT 115 September 2012 STRICTLY CONFIDENTIAL - FOR COMPANY USE ONLY Cooling Technology How does vapor-compression refrigeration work? Condenser warm side high pressure Waste heat Chamber Evaporator cold side low pressure Cold air Throttle Valve refrigerant liquid refrigerant vapor Compressor

Incubators including Peltier KT 115 September 2012 STRICTLY CONFIDENTIAL - FOR COMPANY USE ONLY 18  Peltier element  Works as a heat pump, releasing heat on one side and absorbing heat on the other side.  Effective heat sinks are mandatory. current flow waste heat cold air – + chamber 2.2Cooling Technology How does thermoelectric cooling work?

Incubators including Peltier KT 115 September 2012 STRICTLY CONFIDENTIAL - FOR COMPANY USE ONLY Vapor-compression Cooling Well-known application areas  Fridge (Refrigerator)  Freezer  Air conditioning

Incubators including Peltier KT 115 September 2012 STRICTLY CONFIDENTIAL - FOR COMPANY USE ONLY Thermoelectric Cooling Broad application areas 1  Portable cooler (e.g. camping use)  Air dehumidifier  CPU cooler for high-performance computers  Small laboratory instruments  Diode laser  Thermocycler for PCR (molecular biology labs)  Sensor cooler in digital cameras (e.g. for astro photography) GeneAmp ® PCR System 9700 from Applied Biosystems ® 2 1 Wikipedia 2 products,invitrogen.com

Incubators including Peltier KT 115 September 2012 STRICTLY CONFIDENTIAL - FOR COMPANY USE ONLY Peltier vs. Compressor Technology Distinctively different with convincing features  Advantages of the Compressor  Greater heat compensation  Extended temperature range to sub-zero  Higher cooling-down rates  High cooling efficiency  Advantages of the Peltier-Technology  Safe (no flammable refrigerants)  Eco-friendly (compliant to Roche K6 directive)  Smaller and lighter (see right)  Silent and gentle (less noise and vibrations)  More robust and service-friendly  Less expensive KB 115 Compressor (9.000 g) KT 115 Peltier-Chip (25 g) BINDER Pen (14 g)

Incubators including Peltier KT 115 September 2012 STRICTLY CONFIDENTIAL - FOR COMPANY USE ONLY 22 The BINDER Commitment  Best products  Best services  Best consulting