January 2004 SundayMondayTuesdayWednesdayThursdayFridaySaturday Mice arrive□ Mite spray □ Med feed Mice arrive □ Normal feed Mice arrive Mice arrive Contacts arrive □ Group house contacts □ Mite spray □ Normal feed □ Mice arrive □ Mite spray □ Med feed □ Add/assign contacts Contact testing 20□ Mite spray □ Normal feed □ Mite spray □ Normal feed □ Move contacts 22 Results expected Mice released27□ Mite spray □ Med feed □ Separate contacts Group 1-January (to arrive 1/6-1/14) Mite spray= treat all mice (see SOP) with mite spray weekly Med feed= use fenbendazole-medicated feed (usually weeks 2,4 and 6) Contacts= contact sentinel mice; moved to new cage once weekly, first 2 weeks, then separated into individual cages and used as a standard (bedding) sentinel Testing= diagnostic lab testing (serology, parasitology) Results= serology results (from Charles River Laboratories) Release= mice released from quarantine; mice ready for transfer to colony See Mouse Quarantine-Tech SOP for more detailed information on standard operating procedures for mouse quarantine

February 2004 SundayMondayTuesdayWednesdayThursdayFridaySaturday 1 2 Mice arrive□ Mite spray □ Normal feed Mice arrive □ Normal feed Mice arrive67 89 Contacts arrive □ Group house contacts □ Mite spray □ Med feed Mice arrive □ Normal feed □ Add/assign contacts Mice arrive □ Mite spray □ Normal feed □ Mite spray □ Med feed □ Move contacts Contact testing 24□ Mite spray □ Normal feed □ Mite spray □ Normal feed □ Separate contacts 26 Results expected Mice released Group 2-February (to arrive 2/3-2/12) Mite spray= treat all mice (see SOP) with mite spray weekly Med feed= use fenbendazole-medicated feed (usually weeks 2,4 and 6) Contacts= contact sentinel mice; moved to new cage once weekly, first 2 weeks, then separated into individual cages and used as a standard (bedding) sentinel Testing= diagnostic lab testing (serology, parasitology) Results= serology results (from Charles River Laboratories) Release= mice released from quarantine; mice ready for transfer to colony See Mouse Quarantine-Tech SOP for more detailed information on standard operating procedures for mouse quarantine

March 2004 SundayMondayTuesdayWednesdayThursdayFridaySaturday 1 Mice arrive□ Mite spray □ Med feed Mice arrive □ Normal feed Mice arrive56 78 Contacts arrive □ Group house contacts □ Mite spray □ Normal feed Mice arrive □ Normal feed □ Add/assign contacts Mice arrive □ Mite spray □ Med feed □ Mite spray □ Med feed □ Move contacts Contact testing 23□ Mite spray □ Normal feed □ Mite spray □ Normal feed □ Separate contacts 25 Results expected Mice released30□ Mite spray □ Med feed Group 3-March (to arrive 3/2-3/11) Mite spray= treat all mice (see SOP) with mite spray weekly Med feed= use fenbendazole-medicated feed (usually weeks 2,4 and 6) Contacts= contact sentinel mice; moved to new cage once weekly, first 2 weeks, then separated into individual cages and used as a standard (bedding) sentinel Testing= diagnostic lab testing (serology, parasitology) Results= serology results (from Charles River Laboratories) Release= mice released from quarantine; mice ready for transfer to colony See Mouse Quarantine-Tech SOP for more detailed information on standard operating procedures for mouse quarantine

April 2004 SundayMondayTuesdayWednesdayThursdayFridaySaturday Mice arrive□ Mite spray □ Normal feed Mice arrive □ Normal feed Mice arrive Mice arrive Contacts arrive □ Group house contacts □ Mite spray □ Med feed Mice arrive □ Normal feed □ Add/assign contacts Mice arrive Contact testing 20□ Mite spray □ Normal feed □ Mite spray □ Med feed □ Move contacts 22 Results expected Mice released27□ Mite spray □ Normal feed □ Separate contacts 2930 Group 4-April (to arrive 4/6-4/15) Mite spray= treat all mice (see SOP) with mite spray weekly Med feed= use fenbendazole-medicated feed (usually weeks 2,4 and 6) Contacts= contact sentinel mice; moved to new cage once weekly, first 2 weeks, then separated into individual cages and used as a standard (bedding) sentinel Testing= diagnostic lab testing (serology, parasitology) Results= serology results (from Charles River Laboratories) Release= mice released from quarantine; mice ready for transfer to colony See Mouse Quarantine-Tech SOP for more detailed information on standard operating procedures for mouse quarantine

May 2004 SundayMondayTuesdayWednesdayThursdayFridaySaturday 1 23 Mice arrive□ Mite spray □ Med feed Mice arrive □ Normal feed Mice arrive Mice arrive Contacts arrive □ Group house contacts □ Mite spray □ Normal feed Mice arrive □ Normal feed □ Add/assign contacts Mice arrive Contact testing 18□ Mite spray □ Med feed □ Mite spray □ Med feed □ Move contacts 20 Results expected Mice released25□ Mite spray □ Normal feed □ Separate contacts Group 5-May (to arrive 5/4-5/13) Mite spray= treat all mice (see SOP) with mite spray weekly Med feed= use fenbendazole-medicated feed (usually weeks 2,4 and 6) Contacts= contact sentinel mice; moved to new cage once weekly, first 2 weeks, then separated into individual cages and used as a standard (bedding) sentinel Testing= diagnostic lab testing (serology, parasitology) Results= serology results (from Charles River Laboratories) Release= mice released from quarantine; mice ready for transfer to colony See Mouse Quarantine-Tech SOP for more detailed information on standard operating procedures for mouse quarantine

June 2004 SundayMondayTuesdayWednesdayThursdayFridaySaturday Mice arrive□ Mite spray □ Med feed Mice arrive □ Normal feed Mice arrive45 67 Contacts arrive □ Group house contacts □ Mite spray □ Normal feed Mice arrive □ Normal feed □ Add/assign contacts Mice arrive Contact testing 15□ Mite spray □ Med feed □ Mite spray □ Med feed □ Move contacts 17 Results expected Mice released22□ Mite spray □ Normal feed □ Separate contacts □ Mite spray □ Med feed Group 6-June (to arrive 6/1-6/10) Mite spray= treat all mice (see SOP) with mite spray weekly Med feed= use fenbendazole-medicated feed (usually weeks 2,4 and 6) Contacts= contact sentinel mice; moved to new cage once weekly, first 2 weeks, then separated into individual cages and used as a standard (bedding) sentinel Testing= diagnostic lab testing (serology, parasitology) Results= serology results (from Charles River Laboratories) Release= mice released from quarantine; mice ready for transfer to colony See Mouse Quarantine-Tech SOP for more detailed information on standard operating procedures for mouse quarantine

July 2004 SundayMondayTuesdayWednesdayThursdayFridaySaturday Mice arrive□ Mite spray □ Normal feed Mice arrive □ Normal feed Mice arrive Contact testing Mice arrive Contacts arrive □ Group house contacts □ Mite spray □ Med feed Mice arrive □ Normal feed □ Add/assign contacts Mice arrive Results expected Mice released20□ Mite spray □ Med feed □ Move contacts □ Mite spray □ Normal feed □ Separate contacts Group 7-July (to arrive 7/6-7/15) Mite spray= treat all mice (see SOP) with mite spray weekly Med feed= use fenbendazole-medicated feed (usually weeks 2,4 and 6) Contacts= contact sentinel mice; moved to new cage once weekly, first 2 weeks, then separated into individual cages and used as a standard (bedding) sentinel Testing= diagnostic lab testing (serology, parasitology) Results= serology results (from Charles River Laboratories) Release= mice released from quarantine; mice ready for transfer to colony See Mouse Quarantine-Tech SOP for more detailed information on standard operating procedures for mouse quarantine

August 2004 SundayMondayTuesdayWednesdayThursdayFridaySaturday 12 Mice arrive□ Mite spray □ Med feed Mice arrive □ Normal feed Mice arrive67 89 Contacts arrive □ Group house contacts □ Mite spray □ Normal feed Mice arrive □ Normal feed □ Add/assign contacts Mice arrive Contact testing 17□ Mite spray □ Med feed □ Mite spray □ Med feed □ Move contacts 19 Results expected Mice released24□ Mite spray □ Normal feed □ Separate contacts Group 8-August (to arrive 8/3-8/12) Mite spray= treat all mice (see SOP) with mite spray weekly Med feed= use fenbendazole-medicated feed (usually weeks 2,4 and 6) Contacts= contact sentinel mice; moved to new cage once weekly, first 2 weeks, then separated into individual cages and used as a standard (bedding) sentinel Testing= diagnostic lab testing (serology, parasitology) Results= serology results (from Charles River Laboratories) Release= mice released from quarantine; mice ready for transfer to colony See Mouse Quarantine-Tech SOP for more detailed information on standard operating procedures for mouse quarantine

September 2004 SundayMondayTuesdayWednesdayThursdayFridaySaturday □ Mite spray □ Med feed Mice arrive□ Mite spray □ Normal feed Mice arrive □ Normal feed Mice arrive Contact testing Mice arrive Contacts arrive □ Group house contacts □ Mite spray □ Med feed Mice arrive □ Normal feed □ Add/assign contacts Mice arrive Results expected Mice released21□ Mite spray □ Med feed □ Move contacts □ Mite spray □ Normal feed □ Separate contacts 30 Group 9-September (to arrive 9/7-9/16) Mite spray= treat all mice (see SOP) with mite spray weekly Med feed= use fenbendazole-medicated feed (usually weeks 2,4 and 6) Contacts= contact sentinel mice; moved to new cage once weekly, first 2 weeks, then separated into individual cages and used as a standard (bedding) sentinel Testing= diagnostic lab testing (serology, parasitology) Results= serology results (from Charles River Laboratories) Release= mice released from quarantine; mice ready for transfer to colony See Mouse Quarantine-Tech SOP for more detailed information on standard operating procedures for mouse quarantine

October 2004 SundayMondayTuesdayWednesdayThursdayFridaySaturday Mice arrive□ Mite spray □ Med feed Mice arrive □ Normal feed Mice arrive Mice arrive Contacts arrive □ Group house contacts □ Mite spray □ Normal feed Mice arrive □ Normal feed □ Add/assign contacts Mice arrive Contact testing 19□ Mite spray □ Med feed □ Mite spray □ Med feed □ Move contacts 21 Results expected Mice released26□ Mite spray □ Normal feed □ Separate contacts Group 10-October (to arrive 10/5-10/14) Mite spray= treat all mice (see SOP) with mite spray weekly Med feed= use fenbendazole-medicated feed (usually weeks 2,4 and 6) Contacts= contact sentinel mice; moved to new cage once weekly, first 2 weeks, then separated into individual cages and used as a standard (bedding) sentinel Testing= diagnostic lab testing (serology, parasitology) Results= serology results (from Charles River Laboratories) Release= mice released from quarantine; mice ready for transfer to colony See Mouse Quarantine-Tech SOP for more detailed information on standard operating procedures for mouse quarantine

November 2004 SundayMondayTuesdayWednesdayThursdayFridaySaturday 1 Mice arrive□ Mite spray □ Med feed Mice arrive □ Normal feed Mice arrive56 78 Contacts arrive □ Group house contacts □ Mite spray □ Normal feed Mice arrive □ Normal feed □ Add/assign contacts Mice arrive Contact testing 16□ Mite spray □ Med feed □ Mite spray □ Med feed □ Move contacts 18 Results expected Mice released23□ Mite spray □ Normal feed □ Separate contacts Group 11-November (to arrive 11/2-11/11) Mite spray= treat all mice (see SOP) with mite spray weekly Med feed= use fenbendazole-medicated feed (usually weeks 2,4 and 6) Contacts= contact sentinel mice; moved to new cage once weekly, first 2 weeks, then separated into individual cages and used as a standard (bedding) sentinel Testing= diagnostic lab testing (serology, parasitology) Results= serology results (from Charles River Laboratories) Release= mice released from quarantine; mice ready for transfer to colony See Mouse Quarantine-Tech SOP for more detailed information on standard operating procedures for mouse quarantine

December 2004 SundayMondayTuesdayWednesdayThursdayFridaySaturday □ Mite spray □ Med feed Mice arrive□ Mite spray □ Normal feed Mice arrive □ Normal feed Mice arrive Contact testing Mice arrive Contacts arrive □ Group house contacts □ Mite spray □ Med feed Mice arrive □ Normal feed □ Add/assign contacts Mice arrive Results expected Mice released21□ Mite spray □ Med feed □ Move contacts □ Mite spray □ Normal feed □ Separate contacts 3031 Group 12-December (to arrive 12/7-12/16) Mite spray= treat all mice (see SOP) with mite spray weekly Med feed= use fenbendazole-medicated feed (usually weeks 2,4 and 6) Contacts= contact sentinel mice; moved to new cage once weekly, first 2 weeks, then separated into individual cages and used as a standard (bedding) sentinel Testing= diagnostic lab testing (serology, parasitology) Results= serology results (from Charles River Laboratories) Release= mice released from quarantine; mice ready for transfer to colony See Mouse Quarantine-Tech SOP for more detailed information on standard operating procedures for mouse quarantine