Suppl Table 1 Suppl.Tab.1. Homogeneity comparison of amino acid sequences of selective Bacterial gene products with the reserved TM1-H5-TM2 sequence of.

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Suppl Table 1 Suppl.Tab.1. Homogeneity comparison of amino acid sequences of selective Bacterial gene products with the reserved TM1-H5-TM2 sequence of Kir6.1 NCBI Ref. Sequence No. Possible functionBacterial species Percent Identity (%) Gene name NP_669853Putative transport proteinYersinia pestis KIM10.31 NP_669263Putative transport proteinYersinia pestis KIM10.22 NP_669190Putative transport proteinYersinia pestis KIM9.63 NP_489358K + channel proteinNostoc sp PCC NP_695956Hypothetical membrane protein with possible transport function Bifidobacterium longum9.25 NP_416061Putative transport proteinEscherichia coli K ydfJ NP_602106Putative integral membrane transport protein Corynebacterium glutamicum NP_669678Putative transport protein Yersinia pestis KIM NP_298715Ion transporter Xylella fastidiosa 9a5c 9 8.4NP_ Putative integral membrane transport protein Streptomyces coelicolor A3(2)10 8.2NP_ Putative ion channel membrane protein Streptomyces coelicolor11 7.8NP_ Putative integral membrane transport protein Streptomyces coelicolor A3(2) NP_ Ion transporter Xanthomonas axonopodis NP_ K + -transporting ATPase A chain Agrobacterium tumefaciens str. C NP_416051Putative transport proteinEscherichia coli K1215ydeE 7.0NP_ Putative transport protein Escherichia coli K1216 ybbW 7.0NP_ Transport protein Xanthomonas axonopodis17 6.9NP_668944Putative ion transport proteinYersinia pestis KIM NP_ Putative K + channel protein Helicobacter pylori J NP_624744Putative membrane transport proteinStreptomyces coelicolor A3(2)20 5.5NP_ K + -transporting ATPase B chainClostridium acetobutylicum NP_ K + -transporting ATPase B chainXanthomonas campestris

Suppl Table 2 Suppl.Tab.2. Homogeneity comparison of amino acid sequences of selective bacterial gene products with the reserved TM1-H5-TM2 sequence of Kir6.2 NCBI Ref. Sequence No. Possible functionBacterial species Percent Identity (%) Gene name NP_629116Putative integral membrane transport protein Streptomyces coelicolor A3(2)10.91 NP_669190Putative transport proteinYersinia pestis KIM9.52 NP_627203Putative integral membrane transport protein Streptomyces coelicolor A3(2)9.53 NP_669263Putative transport proteinYersinia pestis KIM9.34 NP_416051Putative transport proteinEscherichia coli K NP_602106Putative integral membrane transport protein Corynebacterium glutamicum9.06 ydeE NP_628015Putative ion channel membrane protein Streptomyces coelicolor NP_415044Putative transport protein Escherichia coli K NP_350262K + -transporting ATPase B chain Clostridium acetobutylicum NP_ Putative membrane transport proteinStreptomyces coelicolor A3(2) NP_ Putative transport protein Escherichia coli K NP_642608Transport protein Xanthomonas axonopodis NP_ Ion transporter Xylella fastidiosa 9a5c NP_ Putative K + channel protein Helicobacter pylori J NP_534284K + -transporting ATPase A chainAgrobacterium tumefaciens str. C NP_ Ion transporter Xanthomonas axonopodis16 7.3NP_ K + channel protein Nostoc sp PCC NP_669853Putative transport proteinYersinia pestis KIM NP_ Putative transport protein Yersinia pestis KIM NP_668944Putative ion transport proteinYersinia pestis KIM NP_ K + -transporting ATPase B chainXanthomonas campestris NP_ Hypothetical membrane protein with possible transport function Bifidobacterium longum ybbW ydfJ

Suppl Table 3 Suppl.Tab.3. Amino acid sequence of TM1-H5-TM2 region between rvKir6.1/rvKir6.2 and the selective E. coli K12 genes Gene Name TM1 domain H5-GFG Motifs TM2 domain INAMLGCIQNIVGL ybbW ydfJ ydeE No match126INA186* --- LGL7 No match MFGC336 IVG43 No match62 IGA rvKir (28 aa) (3 aa) (22 aa) rvKir (28 aa) (3 aa) (22 aa) *amino acid sequence order