142.7 46.6% 64.5 53.9% 246.9 97.0% TcFc1: Expression of CD3, 4 and 8 T cells were gated according to size and granularity to exclude non-vital cells in.

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% % % TcFc1: Expression of CD3, 4 and 8 T cells were gated according to size and granularity to exclude non-vital cells in the FSC/SSC plot. In the histogram analysis, only gated T cells on day 37 of stimulation with Fc1 are presented. Shown are stainings with anti-CD3, anti-CD4 and anti-CD8.

Unstained + sec. Antibody (anti-mouse Ig-FITC) + anti-HLA-A2 (BB7.2) Colo060H: Expression of HLA-A2 Colo60H is a low-passage cell line with a mixed phenotype. The gate R1 is here on the more granular and larger cells. In the histogramm plot, only cells in R1 are shown.

HCT116: Expression of MHC-I and HLA-A2 +/- 48h IFN-g treatment (200IU) Unstained + sec. Antibody (anti-mouse Ig-FITC) + anti-HLA-A2 (BB7.2) without IFN-g + anti-HLA-A2 (BB7.2) + IFN-g Unstained + anti-HLA-ABC-FITC without IFN-g + anti-HLA-ABC-FITC + IFN-g