Supporting Information Figure 1. Venn diagrams displaying the technical triplicate protein group identification for (a) SW480 control (b) SW480 5-FU (c)

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Supporting Information Figure 1. Venn diagrams displaying the technical triplicate protein group identification for (a) SW480 control (b) SW480 5-FU (c) SW260 control (d) SW620 5-FU. Total number of protein groups identified for each technical replicate (TR) is displayed. Supporting Information Figure 2. Representative LC-MS/MS peptide ion fragmentation spectra of select peptides from several proteins (a) Catenin beta-1 (P35222) (b) Pyruvate kinase (P14618) (c) Peroxiredoxin-6 (P30041) (d) Transforming protein RhoA (P61586). Supporting Information Figure 3. The coefficient of variation (CV%) for the proteins identified in each sample are plotted as a function of spectral counts (SpC); (a) SW480 control (b) SW480 5-FU (c) SW620 control (d) SW620 5-FU.

TR 1 TR 2 TR B TR 1 TR 2 TR A TR 1 TR 2 TR C TR 1 TR 2 TR D Supporting Information Figure 1

Supporting Information Figure 2 GDLGIEIPAEK found in KPYM b HFCPNVPIILVGNK found in RhoA d LPFPIIDDR found in PRDX6 c LLNDEDQVVVNK found in CTNNB1 a

a Supporting Information Figure 3