Genetic Engineering
Selective Breeding Humans use selective breeding to pass desired traits on to the next generation of organisms Hybridization – The crossing of dissimilar individuals to bring together the best of both organisms. –Luther Burbank and his amazing Burbank potato. Inbreeding is the continued breeding of individuals with similar characteristics.
Increasing Variation Mutations can be induced to help create variation in organisms. Extra chromosomes (polyploid) are bad for animals (Downs Syndrome) but good for plants. It usually makes them stronger.
Manipulating DNA Genetic Engineering is the process of reading and changing DNA sequences in an organism. Reading the Genetic Code –DNA extraction –Cutting and Labeling DNA –Separating DNA –Reading the DNA sequence –Making Copies (PCR – polymerase chain reaction) We do these same things when we are getting ready to do _________________.
PCR – Polymerase Chain Reaction Making copies to work with.
DNA Fingerprinting
Vocab Plasmid - circular DNA molecule found in bacteria Genetic marker - gene that makes it possible to distinguish bacteria that carry a plasmid with foreign DNA from those that don’t Recombinant DNA – DNA that has been created artificially. DNA from two or more sources is incorporated into a single recombinant molecule. Transgenic organism - term used to refer to an organism that contains genes from other organisms
Transforming Bacteria
Transforming Plants
Transforming Animal Cells Can be transformed similar to plants. Some eggs are large enough to physically inject new DNA by hand. Which can “Knock Out” a gene Transgenic organisms contain genes from other organisms. Making onions glow using jellyfish DNA. Using bacteria to make human insulin. Using genetic modification to improve food supply known as GM foods.
Transgenic Organisms - term used to refer to an organism that contains genes from other organisms Transgenic Bacteria Transgenic Plants Transgenic animals Produce clotting factors insulin HGH Stronger plants More production Pest resistance More production
Cloning member of a population of genetically identical organisms produced from a single cell
“Dolly” “Dolly” was an important break through not just because she was a mammal. Frogs were cloned back in 1950’s Why was dolly so special? –Research and answer this question for me.
Human Genome Project The Human Genome Project is an attempt to sequence all human DNA.