DNA Replication Copying the genetic code. DNA Replication  Unpack the Chromosomes  Unzip the DNA  Base pairing at the replication fork  Leading strand.

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Presentation transcript:

DNA Replication Copying the genetic code

DNA Replication  Unpack the Chromosomes  Unzip the DNA  Base pairing at the replication fork  Leading strand vs Lagging strand

Making Copies

copycopied during cell division Sister Chromatids (identical copies)

Accessing the DNA inactive DNA (deactivated or mitotic) DNA in use

Accessing the DNA

DNA Structure

Complementary Base Pairing

Identical Parent and Daughter Strands

Replication Bubbles helicase 5’ 3’ 5’

Replication Bubbles 5’ 3’ 5’

Replication Bubbles 5’ 3’ 5’

Replication Bubbles copies Replication Fork 5’ 3’ 5’

Replication Bubbles

Releasing the Tension

 Topoisomerase cleaves and uncoils DNA ahead of helicase  Single-stranded DNA binding proteins (SSB) prevent DNA from coiling back up

DNA Replication Primase RNA primer DNA Polymerase III 5’ 3’ 5’

DNA Replication  The leading strands grow toward the replication fork Leading Strands 5’ 3’ 5’

DNA Replication 5’ 3’ 5’

DNA Replication Lagging Strands  The lagging strands grow away from the replication fork 5’ 3’ 5’

DNA Replication 5’ 3’ 5’ DNA Polymerase I DNA Ligase

DNA Repliaction 5’ 3’ 5’

DNA Repliaction Okazaki Fragments 5’ 3’ 5’