Cell Membrane Cell and Molecular Biology Advanced Higher Biology Unit One.

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Cell Membrane Cell and Molecular Biology Advanced Higher Biology Unit One

Learning Objectives To identify the components of the plasma membrane To explain the nature of the plasma membrane using the fluid mosaic model. To list the mechanisms of transport through the cell membrane. To explain the functions of membrane proteins To explain the structure and function of the cytoskeleton.

Experimental work on plasma membrane Damage to cell membrane kills the cell Oil soluble substrates can penetrate quickly => contains lipid Lipid solvents alter permeability properties Theory – Monolayer? – But water in contact with both surfaces, bimolecular – Proteins added to model to explain its strength

Davson and Danielli1935 Lipid bilayer coated on both sides with a layer of protein molecules Calculated that thickness of membrane was about 7.5nm. Phospholipid bilayer Protein molecule

Singer and Nicholson 1972 Fluid mosaic model Cell membranes have a consistency like oil, with sideways movement of molecules or membrane. – Fluid – individual phospholipid and protein molecules move around within their layer – Mosaic– pattern produced by scattered protein molecules when surface membrane is viewed from above.

Fluid Mosaic Model A phospholipid bilayer Transmembrane and peripheral proteins Cholesterol Glycoproteins Glycolipid

Cell Surface Membrane

Functions of the plasma membrane Providing a selectively permeable barrier Compartmentalisation Localising reactions in a cell Transport of solutes Signal transduction Cell-cell recognition Intercellular junctions (desmosome, tight junction, gap junction)

Roles of Components of Membrane Phospholipid – If phospholipid molecules are spread over water they form a single layer with their heads in water. – If shaken up with water they can form stable structures called micelles. – Can form sheets (bilayer) – Form membrane bound compartments. – Act as a barrier to most water soluble substances

Roles of Components of Membrane Cholesterol – Helps regulate fluidity of membrane – Stabilises phospholipid bilayer – Prevent ions/polar molecules passing through, important in myelin sheath around nerve cells.

Roles of Components of Membrane Proteins – Intrinsic proteins – span membrane – Extrinsic proteins – embedded in one half of membrane – Channel forming proteins – Carrier protein molecules

Roles of Components of Membrane Glycolipids and Glycoproteins – Short carbohydrate chains attach to lipids and proteins – Chains project into watery fluid surrounding membrane, forming hydrogen bonds with the water, and increasing stability of membrane structure. – Receptor molecules – bind hormones and neurotransmitters, e.g. insulin receptors in liver and muscle cells – Antigens – allow cells to recognise each other e.g. white blood cells