Rahul Joseph Lee Laim

 The incoming light is in-elastically scattered by the vibration of the molecules.  This change in frequency of the light due to vibrational scattering is the Raman effect.

 Transitions occur in seconds  Raman Shift (cm -1 )  Excess energy released as heat

 Sample volume can be as small as 1µm diameter  Imaging modes Direct Imaging – Imaging over small range of wavenumbers Hyperspectral Imaging –Location profile of various components

 Detect counterfeit drugs  Non-destructive detection of biological components  Monitors gas mixtures in medical applications  Characterization of materials and orientation  Monitors activity in catalytic reactions

6W Argon Ion Laser  nm, nm,, nm, nm, nm, nm.

Laser Light Path

Photomultiplier Tube

Spectrometer (monochrometer) nλ=2dsinΘ

Dye Laser