IMT’s Food QA/QC Assays Andy Moreno Vice President, Microbiology Sales Engineering Cell: FAX: Linkedin Profile SKYPE: Andy Moreno USA Linkedin Profile Slide 1 of 8

Industrial Microbial Testing (IMT) Manufactures assays to support rapid real-time quantitative Polymerase Chain Reaction microbial testing. Actionable Information: Rapid Release/99% product safe Establish Positive Release Program Food Safety Screening: Hold/Release time – ~One (1) hour/PCR vs. Traditional Culture Media Testing – Ingredients, In-process Solutions, Finished Product Sensitivity: One (1) cell per 5 µl of sample One (1) sample, many tests Slide 2 of 8

Real-Time Data Standard Curve Traditional Culture Media vs. ~1 HOUR qRT-PCR versus 3 to 14 days~ One (1) Hour Slide 3 of 8

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“Organism Prospecting Screens” Water or Matrix Irrigation water/Ingredients/In Process/Final Product sample Total Bacterial Count (TBC) screen Is there bacteria? Presence/Absence Quantitation Total Yeasts and Molds Count (YMC) screen Are there yeasts and molds? Presence/Absence Quantitation Slide 5 of 8

IMT’s OneStep Assays 85 µl molecular grade water added to primers with PCR chemistry in dried-down format 5 µl purified DNA Sample per Cepheid Tube plus 20µl water and primer chemistries: total 25 µl—4 test batch ~30 minute run time Slide 6 of 8

Typical Food QA Station & Team IMT TBC Assay and Cepheid SmartCycler Minimally-trained QA/QC team(s) 30 minute, easy-to-use, accurate DNA results Slide 5 of 7

IMT Assays Total Bioburden Assays – OneStep Total Bacteria Count Assay – OneStep Total Yeasts & Molds Count Assay Commercialized Pathogen Assays – E. coli spp. Assay – Salmonella spp. Assay – C. botulinum “E” Assay – Porcine Assay R&D Pipeline – O157:H7 – Campylobacter Assay – Listeria mono. Assay – Enterococcus spp. Assay – Staphylococcus spp. Assay – Pseudomonas spp. Assay – Xanthomonas spp. Assay – Mycoplasma spp. Assay Slide 8 of 8 Samples Total Bacteria Count Pathogen of Interest Positive Release Program

IMT’s Assays Validate Food Producer’s Statistical Process Control Program Periodic Testing of Randomized samples against established maximum and minimum standards.

Food: Positive Release Program Irrigation water tested Raw fruit and vegetables are washed and cleaned Peracetic Acid and Chlorine wash bath tested at the end of each run Finished product is tested Environmental samples collected in plant “hot spots” Slide 10 of 8

Sample Environmental Testing* Surface Sample CollectionCollect bacterial samples in filterOne sample, 5 tests -Total Bacterial Count -E. coli spp. -Salmonella spp. -Listeria spp. Collection and preparation of samples = 30 minutes Quantitative real-time PCR results in 30 minutes *Quantitatively track the presence pathogens in the environment and correlate with spoilage using statistical process control methodologies.