Name:_______________ Quiz 9: Biol 203 Fall 2013 Lab Instructor Name (1pt):_______________ 26pts total A HD R Arl E1E2E3 4pts. Circle the portion of the above gene that you would use to make a transgene that expresses Arl in the leg only. 4pts. Mark with a bracket the portion of the above gene that you would use to make a transgene that expresses Arl in the antenna. 4pts. You want to insert a minimal promoter-Gal4pA transgene near Arl gene. Where is the best place to insert it in order to get leg specific expression and no antenna expression. Using an arrow, point to the location on the Arl gene above. 4pts. You have designed a fancy transgene with a splice acceptor site, the GFP (ATG-stop) gene and a pA. This type of transgene is referred to as a “splice trap”. In order to see GFP expression in the nucleus, where does this transgene need to be incorporated in the above gene?___________________ What else must be true about this incorporation?__________ means enhancer binding site The Arristaless-like (Arl) gene below is expressed in the leg and the antenna. E1 and E2 are necessary and sufficient to drive expression in the leg. E1, E2 and E3 are necessary and sufficient to drive expression in the antenna. If E3 is deleted, no expression of Arl is found in the antenna. Arl is a transcription factor and thus must get into the nucleus to function. However, the nuclear localization sequence is NOT located on exon 1, exon 2, or exon 4. 1) Does the reverse transcribed first-strand cDNA sequence bind to its mRNA in a anti-parallel direction (yes or no)?_______ 2) (all or none) Name (write out names) for the five most common DNA bases________ ________ _______ _________ _________ 3) There are four common RNA bases. However, a fifth type of RNA base is involved in wobble position. Name that base. _________ 4) Pauling and Corey developed a triple helix model that Franklin’s X-ray diffraction data ruled out. What did her data show that ruled out their model?____________________________________ 5) Watson and Crick first tried to pair A with A, G with G, C with C and T with T. Why did they try to make these pairs? ___________________________________________________________________________________________. 6) Donohue ruled out the model of Watson and Crick because_________________________________________. 7) This led Watson and Crick to fix their model in two ways:________________________ and ______________________. Q2-6: 1pt per question, Q1 and 7: 2pts each.

Name:_______________ Quiz 9: Biol 203 Fall 2013 Lab Instructor Name (1pt):_______________ 26pts total A HD R Arl E1E2E3 4pts. Circle the portion of the above gene that you would use to make a transgene that expresses Arl in the antenna only. 4pts. Mark with a bracket the portion of the above gene that you would use to make a transgene that expresses Arl in the leg. 4pts. You want to insert a minimal promoter-Gal4pA transgene near Arl gene. Where is the best place to insert it in order to get leg specific expression and no antenna expression. Using an arrow, point to the location on the Arl gene above. 4pts. You have designed a fancy transgene with a splice acceptor site, the GFP (ATG-stop) gene and a pA. This type of transgene is referred to as a “splice trap”. In order to see GFP expression in the nucleus, where does this transgene need to be incorporated in the above gene?___________________ What else must be true about this incorporation?__________ means enhancer binding site The Arristaless-like (Arl) gene below is expressed in the leg and the antenna. E1 and E2 are necessary and sufficient to drive expression in the leg. E1, E2 and E3 are necessary and sufficient to drive expression in the antenna. If E3 is deleted, no expression of Arl is found in the antenna. Arl is a transcription factor and thus must get into the nucleus to function. However, the nuclear localization sequence is NOT located on exon 1, exon 2, or exon 4. 1) Does the reverse transcribed first-strand cDNA sequence bind to its mRNA in a parallel direction (yes or no)?_______ 2) (all or none) Name (write out names) for the five most common DNA bases________ ________ _______ _________ _________ 3) There are four common RNA bases. However, a fifth type of RNA base is involved in wobble position. Name that base. _________ 4) Pauling and Corey developed a triple helix model that Franklin’s X-ray diffraction data ruled out. What did her data show that ruled out their model?____________________________________ 5) Watson and Crick first tried to pair A with A, G with G, C with C and T with T. Why did they try to make these pairs? ___________________________________________________________________________________________. 6) Donohue ruled out the model of Watson and Crick because_________________________________________. 7) This led Watson and Crick to fix their model in two ways:________________________ and ______________________. Q2-6: 1pt per question, Q1 and 7: 2pts each.