GENETICS & THE WORK OF MENDEL What is Genetics?  Genetics studies heredity.  Heredity is the passing of traits from parents to offspring.

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Presentation transcript:

GENETICS & THE WORK OF MENDEL

What is Genetics?  Genetics studies heredity.  Heredity is the passing of traits from parents to offspring.

Who is Father of Genetics?  Gregor Mendel – in mid-1800s, Austrian monk who was the first person to succeed in predicting how traits passed from parent to offspring  He used garden peas in his experiments.

Mendel’s Experiments  He controlled his experiments to ensure accurate results:  Self-Pollination – pea plant would pollinate itself  Cross-Pollination – one pea plant would pollinate another

Mendel’s Monohybrid Crosses  Mendel selected a white-flower plant and a purple-flower plant.  He crossed them to produce new plants.  Hybrid – offspring of parents that have different forms of a trait (ex. Tall and short height)  Monohybrid – “one trait”

Vocabulary 1. Gene – portion of DNA that codes for a trait or protein 2. Trait – inheritable characteristic 3. Allele – number of alternative forms of the same gene or same genetic locus (spot)

Vocabulary 4. Dominant allele/trait – trumps other alleles (hides them) and is written with an uppercase letter ex. Brown eyes are dominant = B 5. Recessive allele/trait – hidden if dominant allele is present and is written with a lowercase letter ex. Blue eyes are recessive = b

Vocabulary 6. Homozygous – both forms of the allele are the same; also known as purebred, true-breeding ex. Homozygous brown eyes = BB Homozygous blue eyes = bb 7. Heterozygous – forms of the allele are different; also known as hybrid ex. Heterozygous brown eyes = Bb

Pollen transferred from white flower to stigma of purple flower anthers removed all purple flowers result Mendel’s Work F1F1 P F2F2 self-pollinate  Parent generation (P):  White plant x Purple plant  First generation (F 1 ):  Produced all purple plants  F = filial  Second generation (F 2 ):  Self pollinate F 1 generation  Purple plant x purple plant produced 3 purple plants & 1 white plant.

F 2 generation 3:1 75% purple-flower peas 25% white-flower peas Looking closer at Mendel’s work P 100% F 1 generation (hybrids) 100% purple-flower peas X true-breeding purple-flower peas true-breeding white-flower peas self-pollinate

What did Mendel’s findings mean?  Traits come in alternative versions:  Purple vs. White flower color  Alleles - a number of alternative forms of the same gene or same genetic locus (spot) some difference in sequence of A, T, C, G purple-flower allele & white-flower allele are two DNA variations at flower-color locus different versions of gene at same location on homologous chromosomes

What did Mendel’s findings mean?  Some traits mask others:  purple & white flower colors are separate traits that do not blend purple x white ≠ light purple purple masked white  Dominant allele = purple flower color  Recessive allele = white flower color homologous chromosomes Purple Allele Dominant Trait White Allele Recessive Trait

Genotype vs. Phenotype  Difference between how an organism “looks” & its genetics: 1. Phenotype - description of organism’s trait Phenotype = Physical 2. Genotype - description of organism’s genetic makeup Genotype = Genetics Explain Mendel’s results using …dominant & recessive …phenotype & genotype F1F1 P X purplewhite all purple

Making Crosses  Can represent alleles as letters: **when choosing letters, pick letter where uppercase looks different then lowercase  flower color alleles  F or f  true-breeding purple-flower peas  FF  true-breeding white-flower peas  ff FF x ff F1F1 P X purplewhite all purple F F f f Ff

F 2 generation 3:1 75% purple-flower peas 25% white-flower peas ???? Looking closer at Mendel’s work P X true-breeding purple-flower peas true-breeding white-flower peas FF ff 100% F 1 generation (hybrids) 100% purple-flower peas Ff phenotype genotype self-pollinate What are genotypes of F 2 ?

Punnett Squares Ff x Ff Ff male / sperm F f female / eggs Genotype Phenotype FF Ff ff F 1 generation (hybrids) Aaaaah, phenotype & genotype can have different ratios!! FF = 25% Ff = 50% ff = 25% Purple = 75% White = 25% 1:2:1 ratio 3:1 ratio

Mendel’s Laws of Heredity  Law of Segregation:  Alleles for SAME trait separate into different gametes during meiosis.  Ex. Height  Law of Independent Assortment:  Alleles for DIFFERENT traits separate independently of each other during meiosis.  Ex. Blonde hair does not mean you have blue eyes. Traits inherited independently. Tt T t T t

Monohybrid Crosses Practice  Every genetics problem you work must include: 1. Key 2. Cross (Parent x Parent) 3. Punnett Square 4. Results (Genotype & Phenotype)

Monohybrid Crosses Practice 1. Black-colored (B) bear is dominant over brown-colored (b) bear. Cross a homozygous dominant bear with a heterozygous bear. Give phenotypic and genotypic results of F 1 generation. Key B = black b = brown Cross: BB x Bb B B b B BB Bb BB Genotype BB = 50% Bb = 50% 1:1 Ratio Phenotype Black = 100%

Monohybrid Crosses Practice 2. In human, dimples (D) is dominant over no dimples (d). Cross a hybrid woman with a man who does not have dimples. Give phenotypic and genotypic results of F 1 generation. What are the chances the couple will have a child with no dimples? Key D = dimples d = no dimples Cross: Dd x dd D d d d Dd dd Dd dd Genotype Dd = 50% dd = 50% 1:1 Ratio Phenotype Dimples = 50% No dimples = 50% 1:1 Ratio 50% chance of child with no dimples

3. Curly hair (H) is dominant over straight hair (h). Cross a purebred curly haired woman with a true-breeding straight haired man. Give phenotypic and genotypic results of F 1 generation. Then, cross F 1 offspring to show phenotypic and genotypic results of F 2 generation. Key H = curly h = straight Cross: HH x hh H H h h Hh Genotype Hh = 100% Phenotype Curly = 100% H h h H HH hh Hh Genotype HH = 25% Hh = 50% hh = 25% 1:2:1 Ratio Phenotype Curly = 75% Straight = 25% 3:1 Ratio Cross: Hh x Hh Key H = curly h = straight F1F1F1F1 F2F2F2F2