UW BIO LAB NOTEBOOKS LAB 1: BEAUTIFUL BRAINS

LAB NOTEBOOK ALL LAB REPORTS FOR THIS COURSE WILL BE DONE IN A COMPOSITION STYLE LAB NOTEBOOK BE SURE YOUR NAME & PERIOD ARE CLEARLY WRITTEN ON THE COVER/OR INSIDE THE COVER CREATE AN ONGOING TABLE OF CONTENTS ON THE 1 ST PAGE INCLUDE PAGE NUMBERS

TABLE OF CONTENTS LAB1: BEAUTIFUL BRAINS P. ?-?? LAB 2: MEASURING ENZYME ACTIVITY P. ??-?? LAB 3: DNA ISOLATION P. ??-?? LAB 4: PATTERNS OF INHERITANCE P. ??-?? LAB 5: WORMS ON DRUGS P. ??- ?? LAB 6: MEASURING INTOXICATION P.??-?? LAB 7: LIMITS OF PERCEPTION P. ??-??

CEC SUMMARY OF YOUR RESULTS CLAIM: RESTATE THE CLAIM AND STATE WHETHER YOUR HYPOTHESIS WAS ACCEPTED, REJECTED OR INCONCLUSIVE EVIDENCE: SITE SPECIFIC DATA FROM THE DATA TABLE THAT SUPPORTS THE CLAIM. SHOULD INCLUDE HIGH AND LOW VALUES, ETC. COMMENTARY: EXPLAIN WHY THE DATA (EVIDENCE) SUPPORTS THE CLAIM. REVISIONS?

LAB 1: BEAUTIFUL BRAINS SHEEP BRAIN DISSECTION

LEARNING TARGET, SUCCESS CRITERIA & CLAIM LEARNING TARGET BE ABLE TO IDENTIFY ANATOMICAL STRUCTURES IMPORTANT IN THE STUDY OF ADDICTION SUCCESS CRITERIA BE ABLE TO IDENTIFY THE LOCATION AND EXPLAIN THE SIGNIFICANCE OF THE FOLLOWING STRUCTURES: CEREBRAL CORTEX, BRAIN STEM, CEREBELLUM, INSULA, CORPUS CALLOSUM, HYPOTHALAMUS, THALAMUS, HIPPOCAMPUS, MENINGES, & AMYGDALA CLAIM: A SHEEP BRAIN CAN BE USED TO LEARN HUMAN BRAIN ORGANIZATION AND TO BECOME MORE FAMILIAR WITH THE LOCATION OF SPECIFIC BRAIN STRUCTURES IMPORTANT IN UNDERSTANDING DRUG RESPONSE

EXPECTATIONS FOR LAB WRITE-UPS IN THIS COURSE PRE-LAB TO INCLUDE: LEARNING TARGETS MATERIALS NEEDED DETAILED OUTLINE OF THE PROCEDURE LEAVING ROOM FOR LAB OBSERVATIONS/SKETCHES AS INDICATED IN THE PROCEDURE DURING THE LAB COMPLETE LAB OBSERVATIONS/SKETCHES ANSWERS TO ANY LAB PACKET QUESTIONS AFTER THE LAB POST LAB QUESTIONS SUMMARY OF WHAT YOU LEARNED IN THE LAB- WHAT WAS YOU MOST IMPORTANT "AH HA" MOMENT? PRE-LAB MUST BE COMPLETE TO PARTICIPATE IN THE LAB MAKE-UPS MAY NOT BE SCHEDULED, DEPENDING ON THE LAB POST LAB QUIZZES OCCUR TYPICALLY 2 DAYS POST LAB

MATERIALS FOR THIS LAB: BRAIN BOARD/BENCH PAPER SCISSORS SCALPEL (HANDLE WITH CARE: VERY SHARP) GLOVES FORCEPS PROBE SHEEP BRAIN This is what it looks like after the meninges have been removed

PROCEDURE A. WHOLE BRAIN ORGANIZATION WHAT WILL YOU NEED TO LOCATE BEFORE CUTTING THE BRAIN IN HALF? WHAT ARE YOU EXPECTED TO DO? LOCATE THE FOLLOWING: CEREBRAL CORTEX CEREBELLUM MENINGES BRAIN STEM OPTIC CHIASM LOBES: PARIETAL, TEMPORAL, FRONTAL DO THE FOLLOWING: COMPARE SHEEP BRAIN TO HUMAN AS AVAILABLE LOCATE OLFACTORY LOBES IDENTIFY THE SULCCI AND GYRI WHICH ARE THE HILLS & WHICH ARE THE VALLEYS? DESCRIBE THE TEXTURE OF THE CEREBRUM LOCATE CEREBELLUM HOW DOES IT DIFFER FROM THE CEREBRUM WHY IS IT IMPORTANT? NOTE BRAINSTEM POSITION WHY IS THERE A DIFFERENCE COMPARED TO THAT OF A HUMANS

PROCEDURE B. REMOVING THE MENINGES 1.REMOVE THE MENINGES  USE SCISSORS 2.HOLD BRAIN TOP SIDE FACING UP -IDENTIFY EACH HEMISPHERE- -USE SCALPEL TO CUT THROUGH THE CORPUS COLLOSUM -WHAT’S THE FUNCTION OF THE CORPUS COLLOSUM? FIND: CORPUS COLLOSUM VENTRICLES CEREBELLUM THALAMUS LABELED SKETCH OF ONE HEMISPHERE. VIEW 1 (CORPUS COLLOSUM FACING UP): VIEW2: (CORPUS COLLOSUM FACING DOWN)

PROCEDURE DISSECTION PROCEDURE C. WITH ONE HEMISPHERE: IDENTIFY INSULA D. EXAMINE CUT 3 & CUT 4 WHAT % OF BRAIN IS GRAY V. WHITE MATTER? FIND PYRAMIDAL CELLS WHERE GRAY & WHITE MATTER MEET LINKED TO HALLUCINATIONS E. USING OTHER HALF OF BRAIN  CUT CEREBELLUM FREE WHITE MATTER=AXONS

PROCEDURE-EXAMPLE G. ** HARD PART  FIND THE HIPPOCAMPUS 1.FIND DIP WHERE THE OPTIC CHIASM IS (WHERE TEMPORAL LOBE & FRONTAL LOBE MEET) 2.MAKE A HORIZONTAL CUT ONE FINGER WIDTH BEHIND THAT LANDMARK (TOWARD BRAINSTEM) 3.PLACE THUMB ON BRAINSTEM AND THE OTHER ON THE CEREBRUM ROTATE THUMBS APART HIPPOCAMPUS SHOULD POP OUT HIPPOCAMPUS DIAGRAM

HIPPOCAMPUS!

POST LAB QUESTIONS 1.DESCRIBE THE RELEVANCE TO THIS COURSE OF THE FOLLOWING BRAINS PARTS: HIPPOCAMPUS- INSULA- THALAMUS- HYPOTHALAMUS- BRAINSTEM- 2. WHY DID WE HAVE TO DISSECT AN ACTUAL SHEEP BRAIN RATHER THAN LOOK AT PICTURES? 3. WHY DIDN’T WE DISSECT A HUMAN BRAIN? (WHY IS THE SHEEP BRAIN ALMOST AS GOOD?)

CEC SUMMARY OF YOUR RESULTS CLAIM: RESTATE THE CLAIM AND STATE WHETHER YOUR HYPOTHESIS WAS ACCEPTED, REJECTED OR INCONCLUSIVE EVIDENCE: SITE SPECIFIC DATA FROM THE DATA TABLE THAT SUPPORTS THE CLAIM. SHOULD INCLUDE HIGH AND LOW VALUES, ETC. COMMENTARY: EXPLAIN WHY THE DATA (EVIDENCE) SUPPORTS THE CLAIM. REVISIONS?

DISSECTION PROTOCOL MUST HAVE LAB NOTEBOOK WITH PRE-LAB OUT NO FOOD/BEVERAGES OUT- BACK ON COUNTERS PLEASE GROUPS OF 2-3 FINE TO DISSECT AT TABLES… JUST CLEAN THEM WITH CLEANER PROVIDED BE RESPECTFUL OF SPECIMEN & OF YOUR LAB PARTNERS OBTAIN MATERIALS NEEDED FOR GROUP CAUTION!! SCALPELS ARE VERY SHARP! CUT AWAY FROM YOURSELF HUMAN BRAIN DEMO: 26D3DA8FAC0881D 26D3DA8FAC0881D

DISSECTION CLEAN-UP REMOVE SCALPEL, FORCEPS, SCISSORS & PROBE WASH THEM IN PLASTIC CONTAINER WITH SOAPY WATER RINSE, DRY AND RETURN TO BEAKERS DISPOSE OF BRAIN MATTER BY WRAPPING IT UP IN THE BENCH PAPER DISPOSE OF IN RED BIOHAZARD BAG CLEAN OFF THE TABLES PLEASE! MOVE CHAIRS BACK PLEASE!