AIM:
Genetic Engineering: changing the DNA of living organisms. 1. Inserting genes into other organisms 2. Selective Breeding 3. Cloning
Transgenic organism- genes from more than one species Example: GFP mice, silk goats
First, we must ISOLATE the gene Restriction enzymes: cut DNA at specific sequences of nucleotides EcoRI cuts at “sticky end”
Separating DNA Gel electrophoresis can be used to separate DNA sequences
Applications of Transgenic Organisms
Bacteria can produce insulin
Plasmid contains antibiotic resistance= genetic marker
2. Selective Breeding
3. Cloning
Summary: Explain the role of restriction enzymes and plasmids in genetic engineering How could genetic engineering be used to treat medical conditions?