Digestive System & Enzymes 1/29/2016 SWBAT- Discover form(s) and function(s) of the digestive system along with purpose of Enzymes *Students will begin phase one of enzyme lab.

OUT: -HW: Finish Digestive notes from online Calendar In: -DNA-Gel Electrophoresis Viewing -Digestive Parts -Bozeman Video Through: -Notes on Digestive System -Set Up Enzyme Lab OUT: -HW: Finish Digestive notes from online Calendar

Pineapple enzymes BACKGROUND: The enzymes in some tropical fruits can break down protein. For example meat tenderizers have the enzyme, papain, that breaks down some of the fiber. We use it to soften up a tough piece of meat. The papain comes from the papaya fruit. Pineapple has a digestive enzyme called bromelin.

PROCEDURE: With the razor blade carefully cut equal sized pieces of fresh and canned pineapple. The pieces should be just small enough to slide into your cups. Label the cups. Pour 25 ml of liquid gelatin into each of the cups. Place the different pieces of pineapple into the first 2 cups. See the table below to match up the contents. Notice that cup #3 contains only gelatin. Mix the contents of the cups by rolling them upright between the palms of your hands. Place cups into the refrigerator. CUP CONTENTS: 1. Gelatin and Fresh Pineapple 2. Gelatin and Canned Pineapple 3. Gelatin (Mr. Loew’s)

Pineapple enzyme lab (on your own paper) Purpose- What is the question you are trying to answer? Hypothesis- Predict what will happen to the jello.

Observations and analysis Create a table and record 2 observations for each cup. Take 2 observations. One observation today, second observation tomorrow. Analysis Questions: 1. Why did cup #3 (Mr. Loew’s) contain only gelatin? 2. What could account for the different results in the cups? 3. How could the canning process change the enzyme bromelin? 4. Why is it important for pineapple factory workers to wear masks? 5.Design an experiment to find a temperature at which an enzyme works the best? Identify the constants, IV, DV, and control.