Simulation of Protein Identification Through Immunoassay (type of ELISA) Chapter 6: Assays.

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Presentation transcript:

Simulation of Protein Identification Through Immunoassay (type of ELISA) Chapter 6: Assays

What is an immunoprecipitation assay? A test that determines the presence of a unique biochemical in a chemical mixture or biological sample using an antibody/antigen response. A test that determines the presence of a unique biochemical in a chemical mixture or biological sample using an antibody/antigen response.

Antigen vs. Antibody Antibody: Protein produced by the body to recognize and kill foreign pathogens in the body. Antibody: Protein produced by the body to recognize and kill foreign pathogens in the body. Antigen: any substance that causes the body to produce antibodies to kill the pathogen. Antigen: any substance that causes the body to produce antibodies to kill the pathogen.

Uses In Science Determine the presence of disease, evolutionary relationship between different animals. Determine the presence of disease, evolutionary relationship between different animals.

Other Uses of Immunoassay Commonly used by USDA Commonly used by USDA Id components of foods by running tests on diff. proteins to make sure that the foods labeled contain no impurities.Id components of foods by running tests on diff. proteins to make sure that the foods labeled contain no impurities. How do you think this process works?How do you think this process works?

Other Uses of Immunoassay, cont. Home pregnancy tests use this principle to find the hormone human chorionic gonadotropin (hCG) in urine. Home pregnancy tests use this principle to find the hormone human chorionic gonadotropin (hCG) in urine.

NTK Vocabulary Antiserum Antiserum Serum (liquid portion of blood) containing antibodiesSerum (liquid portion of blood) containing antibodies Immunoprecipitation Immunoprecipitation The binding of an antibody to specific antigens forming a white clump called a precipitantThe binding of an antibody to specific antigens forming a white clump called a precipitant Serum Serum Liquid portion of blood. Contains proteins including antibodies that are unique to each animal. All animals have diff types of blood proteins. There are some serum proteins that are specific to the individual—even identical twins could vary!Liquid portion of blood. Contains proteins including antibodies that are unique to each animal. All animals have diff types of blood proteins. There are some serum proteins that are specific to the individual—even identical twins could vary!