Step 3: Electron Transport Chain Location: The inner membrane of the mitochondria Purpose: Use the energy from NADH and FADH 2 to convert ADP into ATP. – NADH and FADH 2 were produced during glycolysis and the Kred’s cycle. – NADH → 3 ATP’s – FADH 2 → 2 ATP’s

The Process 1.NADH and FADH2 give high energy elections to protein pumps in the inner mitochondria membrane. 2.Energy from the electrons is used to pump H+ ions from to matrix to the intermembrane space. 3.There is now a higher concentration of H+ in the intermembrane space compared to the matrix. 4.Chemiosmosis: H+ ions flow back to the matrix through an enzyme called ATP Synthase 5.ATP Synthase: using the kinetic energy from H+ moving down a concentration gradient to convert ADP into ATP Step 3: Electron Transport Chain