PE: Hydration, enthalpy and entropy

Intermediate structures Between Phases

31 P Solid State NMR of lipid assemblies Breath, anisotropy and isotropic chemical shifts

Solution Vs Solid State NMR Fast isotropic tumblingSlow to no molecular reorientation Isotropic chemical shifts narrow lines J couplings Chemical shift powder patterns broad lines dipolar broadening quadrupolar broadening 2 H NMR - quadrupole nuclei 31 P NMR- S=1/2 nuclei

Magnetic Anisotropic Interactions Chemical shielding/chemical shift

Chemical Shift Dependence Upon Orientation Integrate over all space for a “powder” MLVs and GUVs act as powders. They are large enough to tumble slowly enough NOT to average the CSA and 2H quad interaction

Chemical Shift Dependence for Crystals

Dry powder Restricted motions mixtures of different lipids mixtures of different phases exchange rates

Exchange Between Two Sites: Effects on NMR Lineshapes Two states: A and B

Hydration effects (entropic) on L  to H II phase for DOPE