Haemoglobinopathies - Lab Tests Solubility:. Haemoglobinopathies - Lab Tests Solubility: For Hb S Cause the abnormal Hb S to precipitate. Reducing substances.

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Presentation transcript:

Haemoglobinopathies - Lab Tests Solubility:

Haemoglobinopathies - Lab Tests Solubility: For Hb S Cause the abnormal Hb S to precipitate. Reducing substances induce sickling in vitro. Sodium metabisulphite and sodium dithionite False Negative Result Anaemia Post Tx False Positive Result Icteric/lipaemic Sample Other sickling Hb: e.g. Hb Jamaica Plain

Haemoglobinopathies - Lab Tests HPLC 1 Solvent reservoirs, 2 Solvent degasser 3 Gradient valve 4 Mixing vessel 5 High-pressure pump, 6 Switching valve in "inject position", 6' Switching valve in "load position", 7 Sample injection loop, 8 Pre-column (guard column),guard column 9 Analytical column, 10 Detector 11 Data acquisition, 12 Waste

Haemoglobinopathies - Lab Tests HPLC Variant Hb Raised Hb A 2

normal Haemoglobinopathies - Lab Tests

sickle cell trait - AS Haemoglobinopathies - Lab Tests

sickle cell disease - SS Haemoglobinopathies - Lab Tests

Hb C carrier - AC Haemoglobinopathies - Lab Tests

raised Hb A 2 Haemoglobinopathies - Lab Tests

Electrophoresis acid pH alkali pH 2ControlNormal 3ControlAS 4ControlAC 5ControlSS 6Control  -thalassaemia trait (note similarity to 2) 7TestAS 8TestAS 9TestEE

Haemoglobinopathies - Lab Tests Blood Count Data: Hypochromic microcytic (relative) erythrocytosis MCH Target cells (due to membrane damage from globin chains) Sickled cells Crystallised Hb

Haemoglobinopathies - Lab Tests Given a Fresh Sample: Hb H preparation